Detailed information of TA system TA155013

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 850559..851390
• Replicon: chromosome
• Accession: NZ_CP051719
• Organism: Escherichia coli strain SCU-116

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HHJ31_RS04180
• Protein ID: WP_000854814.1
• Coordinates: 851016..851390 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1PWQ3
• Locus tag: HHJ31_RS04175
• Protein ID: WP_001285586.1
• Coordinates: 850559..850927 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ31_RS04140	845776..846846	+	1071	WP_000102631.1	patatin-like phospholipase family protein	-
HHJ31_RS04145	846982..847659	+	678	WP_001362823.1	hypothetical protein	-
HHJ31_RS04150	847675..848085	+	411	WP_000846704.1	hypothetical protein	-
HHJ31_RS04155	848306..849127	+	822	WP_001234569.1	DUF945 domain-containing protein	-
HHJ31_RS04160	849218..849703	+	486	WP_000213703.1	antirestriction protein	-
HHJ31_RS04165	849719..850195	+	477	WP_001351157.1	RadC family protein	-
HHJ31_RS04170	850264..850485	+	222	WP_000692323.1	DUF987 domain-containing protein	-
HHJ31_RS04175	850559..850927	+	369	WP_001285586.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HHJ31_RS04180	851016..851390	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HHJ31_RS04185	851387..851581	+	195	WP_000988601.1	hypothetical protein	-
HHJ31_RS04190	851594..851707	+	114	WP_001161660.1	DUF957 domain-containing protein	-
HHJ31_RS04195	851996..852142	-	147	Protein_820	transposase domain-containing protein	-
HHJ31_RS04200	852196..852378	+	183	WP_001016348.1	hypothetical protein	-
HHJ31_RS04205	852479..852808	-	330	WP_000450409.1	DUF496 family protein	-
HHJ31_RS04210	852980..854038	-	1059	WP_001200890.1	FUSC family protein	-
HHJ31_RS04215	854236..854709	-	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
HHJ31_RS04220	854829..855995	-	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T155013 WP_000854814.1 NZ_CP051719:851016-851390 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T155013 NZ_CP068591:c3856517-3856182 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13563.41 Da
• Isoelectric Point: 5.0468

>AT155013 WP_001285586.1 NZ_CP051719:850559-850927 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT155013 NZ_CP068591:c3856765-3856517 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGAGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: S1PWQ3