Detailed information of TA system TA155002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 18454..19097
• Replicon: plasmid pSCU-118-1
• Accession: NZ_CP051717
• Organism: Escherichia coli strain SCU-118

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: HHJ32_RS22130
• Protein ID: WP_001044768.1
• Coordinates: 18681..19097 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: HHJ32_RS22125
• Protein ID: WP_001261287.1
• Coordinates: 18454..18684 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ32_RS22105	14574..15542	-	969	WP_100620302.1	IS5-like element IS903B family transposase	-
HHJ32_RS22110	15565..16929	+	1365	Protein_14	exclusion suppressor FxsA	-
HHJ32_RS22115	16979..17878	-	900	WP_000963206.1	nucleotide-binding protein	-
HHJ32_RS22120	17868..18158	-	291	WP_000111771.1	hypothetical protein	-
HHJ32_RS22125	18454..18684	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HHJ32_RS22130	18681..19097	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
HHJ32_RS22135	19259..21397	-	2139	WP_000350638.1	AAA family ATPase	-
HHJ32_RS22140	21751..22008	+	258	WP_000343085.1	hypothetical protein	-
HHJ32_RS22145	22008..22598	+	591	WP_000194575.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / qacE / sul1 / mph(B) / sitABCD	-	1..85236	85236

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T155002 WP_001044768.1 NZ_CP051717:18681-19097 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T155002 NZ_CP068591:c2181025-2180918 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT155002 WP_001261287.1 NZ_CP051717:18454-18684 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT155002 NZ_CP068591:2181073-2181139 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTC

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4