Detailed information of TA system TA154994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2235041..2235567
• Replicon: chromosome
• Accession: NZ_CP051716
• Organism: Escherichia coli strain SCU-118

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: HHJ32_RS10715
• Protein ID: WP_000323025.1
• Coordinates: 2235280..2235567 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: HHJ32_RS10710
• Protein ID: WP_000534858.1
• Coordinates: 2235041..2235280 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ32_RS10680	2230700..2230918	-	219	WP_001171942.1	hypothetical protein	-
HHJ32_RS10685	2231078..2231233	-	156	WP_000379575.1	DUF1391 family protein	-
HHJ32_RS22605	2231370..2231657	+	288	Protein_2086	hypothetical protein	-
HHJ32_RS10695	2232426..2233739	+	1314	WP_000589005.1	ISNCY family transposase	-
HHJ32_RS10700	2234176..2234508	-	333	WP_001301033.1	protein FlxA	-
HHJ32_RS10705	2234711..2235016	-	306	WP_001326990.1	hypothetical protein	-
HHJ32_RS10710	2235041..2235280	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
HHJ32_RS10715	2235280..2235567	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
HHJ32_RS10720	2235639..2235794	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
HHJ32_RS10725	2236011..2236262	+	252	WP_000980994.1	hypothetical protein	-
HHJ32_RS10730	2236329..2236607	+	279	WP_012304870.1	hypothetical protein	-
HHJ32_RS10735	2236609..2237658	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
HHJ32_RS10740	2237672..2238424	+	753	WP_001047135.1	antitermination protein	-
HHJ32_RS10745	2238702..2238791	-	90	WP_120795389.1	hypothetical protein	-
HHJ32_RS10750	2238846..2239058	-	213	WP_000087756.1	cold shock-like protein CspF	-
HHJ32_RS10755	2239359..2239574	+	216	WP_000066484.1	cold shock-like protein CspB	-
HHJ32_RS10760	2240328..2240543	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2221517..2250322	28805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T154994 WP_000323025.1 NZ_CP051716:2235280-2235567 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T154994 NZ_CP068576:925106-925468 [Staphylococcus equorum]
ATGATGAGAAGAGGAGACGTTTATTTAGCTGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
TATCATACAGAATGATACTGGAAATAAATATAGTCCTACCGTAATTGTAGCTGCAATTACTGGTAGGATAAATAAAGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGTAAATATAAGCTTGATAAAGATTCTGTAATACTGCTTGAGCAGATTAGA
ACTTTAGATAAAAATAGGCTGAAAGAAAAATTAACATACCTTTCTGAAAGTAAAATGAAAGAAGTTAATGTTGCCATAGA
TATAAGCTTAGGATTACATAATGTTAGAAATAGTAAATCATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT154994 WP_000534858.1 NZ_CP051716:2235041-2235280 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT154994 NZ_CP068576:924939-925109 [Staphylococcus equorum]
ATGGCAAGTTTTAATCAAAGCAGAAACCATAGTATTGAACAACTATTAAAAGAAGGCTATTCACAAATGGCCGATTTAAA
TCTCTCCCTTGCAGCAGAAGCATTTCCATTTGAATGTGAAGCTTGTGATTGCAATGAAGTTTACCTCAGCTCTAAGAATA
ACAATGAATGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8