Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 842677..843331 Replicon chromosome
Accession NZ_CP051716
Organism Escherichia coli strain SCU-118

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HHJ32_RS04080 Protein ID WP_000244777.1
Coordinates 842924..843331 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HHJ32_RS04075 Protein ID WP_000354046.1
Coordinates 842677..842943 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ32_RS04050 837846..838589 + 744 WP_000951964.1 SDR family oxidoreductase -
HHJ32_RS04055 838646..840079 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HHJ32_RS04060 840124..840435 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HHJ32_RS04065 840599..841258 + 660 WP_000250274.1 hemolysin III family protein -
HHJ32_RS04070 841454..842434 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HHJ32_RS04075 842677..842943 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HHJ32_RS04080 842924..843331 + 408 WP_000244777.1 toxin CptA Toxin
HHJ32_RS04085 843371..843892 - 522 WP_001055874.1 flavodoxin FldB -
HHJ32_RS04090 844004..844900 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HHJ32_RS04095 844925..845635 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HHJ32_RS04100 845641..847374 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T154983 WP_000244777.1 NZ_CP051716:842924-843331 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T154983 NZ_CP068572:c3205657-3205555 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT154983 WP_000354046.1 NZ_CP051716:842677-842943 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT154983 NZ_CP068572:3205548-3205693 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References