Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4391741..4392238 Replicon chromosome
Accession NZ_CP051714
Organism Escherichia coli strain SCU-122

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HHJ35_RS21045 Protein ID WP_001173343.1
Coordinates 4391741..4391914 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HHJ35_RS21050 Protein ID WP_001312331.1
Coordinates 4391942..4392238 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ35_RS21035 4388411..4389868 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
HHJ35_RS21040 4390105..4391622 + 1518 WP_000003806.1 lysine--tRNA ligase -
HHJ35_RS21045 4391741..4391914 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HHJ35_RS21050 4391942..4392238 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HHJ35_RS21055 4392466..4392738 - 273 WP_000405647.1 N-acetyltransferase -
HHJ35_RS21060 4392750..4392980 - 231 WP_000373293.1 (4Fe-4S)-binding protein -
HHJ35_RS21065 4393161..4394792 + 1632 WP_001216482.1 sensor histidine kinase -
HHJ35_RS21070 4394789..4395508 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T154978 WP_001173343.1 NZ_CP051714:c4391914-4391741 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T154978 NZ_CP068564:319402-319752 [Keratinibaculum paraultunense]
GTGATCGTAAAAAGAGGAGATGTTTTTTATGCTGATCTGAGTCCGGTAATTGGCTCTGAGCAAGGAGGAGTAAGACCAGT
TCTTGTAATTCAAAATGATATAGGAAATAAATATAGTCCTACTGTTATTATTGCAGCTATTACATCACAAATAAATAAAG
CAAAGTTGCCTACCCATGTTGAAATTAATGCTTCTGAATATGGATTACCTAAAGATTCAGTAGTATTATTAGAGCAAATT
AGAACTATAGATAAAAAGAGACTTCGAGAGAAGATTGGCAGATTTAGTGATGAGATGATGGATAAAGTTGATGAGTGTTT
AAAAATAAGTTTAGGTTTAATAGATTTATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT154978 WP_001312331.1 NZ_CP051714:c4392238-4391942 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT154978 NZ_CP068564:319124-319405 [Keratinibaculum paraultunense]
ATGGCTGAGACTAAGAAGATTACTGTTAGTTTACCAAATAGTTTGATTGAAGAAGTGGATTTTATAGTTGCTATGGAGAA
AAAAAATAGGAGTGAATTTATAAAAGAAGCTATGAAACTATATATTCGTGAAAAACATAAAGTTCAAGTATATAAGCAAC
TTAAGGATGGATATGTGGAAATGAGTAAAATTAACTCAACCCTTGCTGAAGTAGGGCTAGAACAGGATATGGCTGAGTTA
AACGTTTATGAAACAAGGCTTACGGGGTGTGAAAAAGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References