Detailed information of TA system TA154977

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4380155..4380989
• Replicon: chromosome
• Accession: NZ_CP051714
• Organism: Escherichia coli strain SCU-122

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: HHJ35_RS21000
• Protein ID: WP_000854820.1
• Coordinates: 4380612..4380989 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: HHJ35_RS20995
• Protein ID: WP_001285610.1
• Coordinates: 4380155..4380523 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ35_RS20960	4375876..4376556	+	681	WP_021515201.1	WYL domain-containing protein	-
HHJ35_RS20965	4376707..4377384	+	678	WP_001097565.1	hypothetical protein	-
HHJ35_RS20970	4377390..4377623	+	234	WP_000883181.1	DUF905 family protein	-
HHJ35_RS20975	4377713..4378531	+	819	WP_001175155.1	DUF945 domain-containing protein	-
HHJ35_RS20980	4378797..4379276	+	480	WP_000706978.1	antirestriction protein	-
HHJ35_RS20985	4379291..4379767	+	477	WP_001186193.1	RadC family protein	-
HHJ35_RS20990	4379854..4380075	+	222	WP_000692347.1	DUF987 domain-containing protein	-
HHJ35_RS20995	4380155..4380523	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ35_RS21000	4380612..4380989	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HHJ35_RS21005	4380986..4381183	+	198	Protein_4104	hypothetical protein	-
HHJ35_RS21010	4381211..4381408	+	198	WP_085949158.1	DUF957 domain-containing protein	-
HHJ35_RS21015	4381493..4382053	+	561	Protein_4106	DUF4942 domain-containing protein	-
HHJ35_RS21020	4382887..4384425	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T154977 WP_000854820.1 NZ_CP051714:4380612-4380989 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T154977 NZ_CP068563:c1208823-1208077 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCAGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT154977 WP_001285610.1 NZ_CP051714:4380155-4380523 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT154977 NZ_CP068563:c1208076-1207906 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6