Detailed information of TA system TA154976

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4123604..4124438
• Replicon: chromosome
• Accession: NZ_CP051714
• Organism: Escherichia coli strain SCU-122

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: HHJ35_RS19710
• Protein ID: WP_000854770.1
• Coordinates: 4123604..4123981 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HHJ35_RS19715
• Protein ID: WP_001332042.1
• Coordinates: 4124070..4124438 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ35_RS19685	4122128..4122304	-	177	Protein_3846	helix-turn-helix domain-containing protein	-
HHJ35_RS24120	4122668..4122826	-	159	WP_001467148.1	hypothetical protein	-
HHJ35_RS19700	4122926..4123102	-	177	WP_000839286.1	DUF957 domain-containing protein	-
HHJ35_RS19705	4123119..4123607	-	489	WP_000761690.1	hypothetical protein	-
HHJ35_RS19710	4123604..4123981	-	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
HHJ35_RS19715	4124070..4124438	-	369	WP_001332042.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ35_RS19720	4124601..4124822	-	222	WP_000692323.1	DUF987 domain-containing protein	-
HHJ35_RS19725	4124885..4125361	-	477	WP_001557203.1	RadC family protein	-
HHJ35_RS19730	4125377..4125841	-	465	WP_000855061.1	antirestriction protein	-
HHJ35_RS19735	4126183..4127001	-	819	WP_001234738.1	DUF945 domain-containing protein	-
HHJ35_RS19740	4127156..4127314	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4111682..4148214	36532

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T154976 WP_000854770.1 NZ_CP051714:c4123981-4123604 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T154976 NZ_CP068563:478445-478795 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAACGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13677.38 Da
• Isoelectric Point: 6.5931

>AT154976 WP_001332042.1 NZ_CP051714:c4124438-4124070 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT154976 NZ_CP068563:478158-478439 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACGACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9