Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4052020..4052596 Replicon chromosome
Accession NZ_CP051714
Organism Escherichia coli strain SCU-122

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HHJ35_RS19355 Protein ID WP_001297643.1
Coordinates 4052309..4052596 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HHJ35_RS19350 Protein ID WP_000063156.1
Coordinates 4052020..4052322 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ35_RS19335 4048616..4050766 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
HHJ35_RS19340 4050816..4051019 + 204 WP_000467859.1 YbdD/YjiX family protein -
HHJ35_RS19345 4051030..4051986 + 957 WP_001297640.1 GTPase -
HHJ35_RS19350 4052020..4052322 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HHJ35_RS19355 4052309..4052596 - 288 WP_001297643.1 BrnT family toxin Toxin
HHJ35_RS19360 4052795..4053492 - 698 Protein_3780 IS1 family transposase -
HHJ35_RS19365 4053549..4053644 + 96 Protein_3781 dienelactone hydrolase family protein -
HHJ35_RS19370 4053790..4055022 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
HHJ35_RS19375 4055063..4055590 + 528 Protein_3783 DUF445 family protein -
HHJ35_RS19380 4055638..4057175 + 1538 WP_085953974.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4052795..4053088 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T154974 WP_001297643.1 NZ_CP051714:c4052596-4052309 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T154974 NZ_CP068562:2237759-2238103 [Morganella morganii]
GTGTGGACAGTATTATTAACGGATTATTTTAATGAATGGTTGTCAAAACAAGATACCCGGATGAAAGAAAAAGTGCTCGC
ATCTCTTGGCAATCTTGAAATTTATGGCCCTGTTCTGACAAGACCTTATGTTGATACAGTTAAAACTTCTCAGTACCCGA
ATATGAAAGAATTGAGAATTTGGCATGCTGGTCGTGCAATACGTGCATTTTTTGCCTTTGATCCTGAAAGGCAGGCCATT
ATTTTATGTGCCGGAGATAAAGGGAAGGATAAACGATTTTATCAGACGATGATTCGTATTGCGGACGAACAATTTTCCTC
ATATCTTTCAGCAACGCAGGAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT154974 WP_000063156.1 NZ_CP051714:c4052322-4052020 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT154974 NZ_CP068562:2238108-2238407 [Morganella morganii]
ATGAAAACATTACAGGAAGTGATAGCGGCTCAATCACCGGAGAGCCGTGAGCGGATAAAACAACTGACTGATGAGCTGGT
TCTTGAAACCGGACTGCAACTTCTCAGAGAAGAACTTCATATCTCTCAGAAAACGCTGGCAAATAATCTTGGTATATCAC
AGCCGGCTGTTACTCAGATTGAGCAGCGCGGTAATGATATCAAACTGGCAACGCTGAAACGCTATATTGAGGCAATGGGC
GGAACACTTCGTCTGACGATTGAACTACCGACCGGTGATAACCGGATTTTTCGTGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References