Detailed information of TA system TA154966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2489065..2490653
• Replicon: chromosome
• Accession: NZ_CP051714
• Organism: Escherichia coli strain SCU-122

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: HHJ35_RS11835
• Protein ID: WP_001125475.1
• Coordinates: 2489331..2490653 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HHJ35_RS11830
• Protein ID: WP_001332145.1
• Coordinates: 2489065..2489331 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ35_RS11815	2485433..2486884	+	1452	WP_000854637.1	tagaturonate reductase	-
HHJ35_RS11820	2487081..2487995	+	915	WP_001306069.1	bestrophin family protein	-
HHJ35_RS11825	2487999..2488757	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
HHJ35_RS11830	2489065..2489331	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HHJ35_RS11835	2489331..2490653	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HHJ35_RS11840	2490912..2491169	-	258	WP_001210030.1	hypothetical protein	-
HHJ35_RS11845	2491279..2492061	+	783	WP_001221834.1	SDR family oxidoreductase	-
HHJ35_RS11850	2492495..2493058	+	564	WP_001296074.1	type 1 fimbrial major subunit FimA	-
HHJ35_RS11855	2493420..2494130	+	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T154966 WP_001125475.1 NZ_CP051714:2489331-2490653 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T154966 NZ_CP068553:c299210-299058 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGTATATTAGCAAGTTTAGTTGCCAGTTTAATAGTTTACTTAACTAGTAAGTTATTTAAAAA
AGTAAAAAGCCACTCTGGCAGGAGTGACTTTAGTTTTGAACTAGAAATCAAGTTCAAAAAGAATAAACATTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT154966 WP_001332145.1 NZ_CP051714:2489065-2489331 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT154966 NZ_CP068553:299014-299114 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTCTAGTTC
AAAACTAAAGTCACTCCTGCC

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8