Detailed information of TA system TA154957

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1913210..1914041
• Replicon: chromosome
• Accession: NZ_CP051714
• Organism: Escherichia coli strain SCU-122

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HHJ35_RS09090
• Protein ID: WP_000854814.1
• Coordinates: 1913210..1913584 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1PWQ3
• Locus tag: HHJ35_RS09095
• Protein ID: WP_001285586.1
• Coordinates: 1913673..1914041 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ35_RS09050	1908606..1909772	+	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
HHJ35_RS09055	1909891..1910364	+	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
HHJ35_RS09060	1910562..1911620	+	1059	WP_001200890.1	FUSC family protein	-
HHJ35_RS09065	1911792..1912121	+	330	WP_000450409.1	DUF496 family protein	-
HHJ35_RS09070	1912222..1912404	-	183	WP_001016348.1	hypothetical protein	-
HHJ35_RS09075	1912458..1912604	+	147	Protein_1776	transposase domain-containing protein	-
HHJ35_RS09080	1912893..1913006	-	114	WP_001161660.1	DUF957 domain-containing protein	-
HHJ35_RS09085	1913019..1913213	-	195	WP_000988601.1	hypothetical protein	-
HHJ35_RS09090	1913210..1913584	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HHJ35_RS09095	1913673..1914041	-	369	WP_001285586.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HHJ35_RS09100	1914115..1914336	-	222	WP_000692323.1	DUF987 domain-containing protein	-
HHJ35_RS09105	1914405..1914881	-	477	WP_001351157.1	RadC family protein	-
HHJ35_RS09110	1914897..1915382	-	486	WP_000213703.1	antirestriction protein	-
HHJ35_RS09115	1915473..1916294	-	822	WP_001234569.1	DUF945 domain-containing protein	-
HHJ35_RS09120	1916515..1916925	-	411	WP_000846704.1	hypothetical protein	-
HHJ35_RS09125	1916941..1917618	-	678	WP_001362823.1	hypothetical protein	-
HHJ35_RS09130	1917754..1918824	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T154957 WP_000854814.1 NZ_CP051714:c1913584-1913210 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T154957 NZ_CP068547:c1922064-1921780 [Tamlana sp. s12]
ATGGGAACTTCAAGATTTGAAATAGCAGATTATTTAGAAAGCAAAGAAATGATTGCAGAATACCTCAATACTGTTTTAGA
AGAGGGGACAAATGCAGATATCATTAATGCCATCGGACATATCGCAAAAGCTATAGGAATGACAAAAATTTCAGAGGAAA
CTGGTTTGAGTAGACCAAGTTTATATAAAGCATTATCTGATGGTGCAAAACCACAATTTGCGACAATTATGAAAGTATTA
AAAGCAATCGGAGGACAAATTCAAATTAATCCTATATCTAGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13563.41 Da
• Isoelectric Point: 5.0468

>AT154957 WP_001285586.1 NZ_CP051714:c1914041-1913673 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT154957 NZ_CP068547:c1922367-1922068 [Tamlana sp. s12]
ATGTTCTTTATTGAAAAAACAAATGAATTTGACAAATGGTTTAGAAAGCTAAAAGACCTAAAAGCGAAAGCTAAAATTTT
ATTCAGAATTCAAAAATTAGAAACCGACGAACATTTTGGAGATTGTAAAATTGTTGGTGATGGTATTCGAGAAATGAGAA
TAAATTTCGCTAAAGGCTATAGGGTTTATTTCAAAGAAAAAGACAGAAAAATAATTGTACTGCTTATAGGCGGGGATAAA
TCTACACAACAAAAAGACATTACAAAAGCAAAAGAAATTTGGAGAAATTTAAACAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: S1PWQ3