Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 953208..953862 Replicon chromosome
Accession NZ_CP051714
Organism Escherichia coli strain SCU-122

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HHJ35_RS04640 Protein ID WP_000244781.1
Coordinates 953455..953862 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HHJ35_RS04635 Protein ID WP_000354046.1
Coordinates 953208..953474 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ35_RS04615 949296..950729 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
HHJ35_RS04620 950774..951085 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
HHJ35_RS04625 951249..951908 + 660 WP_000250274.1 hemolysin III family protein -
HHJ35_RS04630 951985..952965 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
HHJ35_RS04635 953208..953474 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HHJ35_RS04640 953455..953862 + 408 WP_000244781.1 protein YgfX Toxin
HHJ35_RS04645 953902..954423 - 522 WP_001055874.1 flavodoxin FldB -
HHJ35_RS04650 954535..955431 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
HHJ35_RS04655 955456..956166 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HHJ35_RS04660 956172..957905 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T154954 WP_000244781.1 NZ_CP051714:953455-953862 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T154954 NZ_CP068444:c4659095-4658856 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGCGTTTGATCCGCGTGCCTGGCGCGAATGGCATAAGCTTGGCGAGACGATCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCCGCGAATTGCATCGGCCAGTCTGAGTGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGGGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT154954 WP_000354046.1 NZ_CP051714:953208-953474 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT154954 NZ_CP068444:c4659327-4659085 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTGCGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCAGTGAAAAGTATGACTATCAGCGATA
GCGAAGCGGCATTATTACGCACCGTCCGCGAGCGGCTGGAAAACCCGCAGCCGGAGGTGAAGGTAAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References