Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3301021..3301518 Replicon chromosome
Accession NZ_CP051711
Organism Escherichia coli strain SCU-123

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HHJ36_RS15710 Protein ID WP_001173343.1
Coordinates 3301345..3301518 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HHJ36_RS15705 Protein ID WP_001312331.1
Coordinates 3301021..3301317 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ36_RS15685 3297751..3298470 - 720 WP_000611291.1 two-component system response regulator DcuR -
HHJ36_RS15690 3298467..3300098 - 1632 WP_001216482.1 sensor histidine kinase -
HHJ36_RS15695 3300279..3300509 + 231 WP_000373293.1 (4Fe-4S)-binding protein -
HHJ36_RS15700 3300521..3300793 + 273 WP_000405647.1 N-acetyltransferase -
HHJ36_RS15705 3301021..3301317 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HHJ36_RS15710 3301345..3301518 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HHJ36_RS15715 3301637..3303154 - 1518 WP_000003806.1 lysine--tRNA ligase -
HHJ36_RS15720 3303391..3304848 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T154937 WP_001173343.1 NZ_CP051711:3301345-3301518 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T154937 NZ_CP068437:c4944008-4942686 [Shewanella sp. KX20019]
ATGGCTGTTCTAGATGTTTATATGAATGGCTATCTCGTCGGAGGTTTCACTAAATCCACTACGGGTTTCCATCTGTTTCA
GTATGCTGCTCAATGGTTAAGTACACCGAATAGTCGCCCAATCTCTTTGTCTATGCCGCTGCGAAAAAAAGCGTATAAAG
GCGATGAGGTTTATAACTTCTTTGATAATTTACTGCCAGATAATATTGAGGTGAGAAACAGAATTGTTGCTCGCCACAAT
GCGCAATCGACACAAGCATTCGATCTATTAGCTAAAATCGGACAAGACAGTGTAGGGGCGTTACAGTTAGTAGCATGCGG
ACAAAAAGCTTTTGATATTAAAAAAATTGAATCTAAGCCCTTATCTGATACGGCATTGGAAAAAGTACTAAAGGGCTATC
AGTCAGGTATTCCGTTAGGTATGTTAGCGGAAGAAGATGATTTTCGAATTTCTATTGCGGGCGCCCAAGAAAAAACAGCT
TTACTCAATCTAGATGGACAATGGCGCCTTCCTATCAAGGCTACTCCAACAACGCATATTATTAAGCTGCCAATCGGTGA
GATCCAGAGTCATTCACACACCATTGATATGCCAGACAGCGTTGAAAATGAATACCTGTGCATGATGATAGTCAGGGAGT
TTGGCTTAGATGTACCGCACTGCTCAATAATTAGGGTCGGCGATATTAAGGCGCTCGCGGTTGAGAGGTTTGATCGCAGG
TTATCAAGTGATGGCAAATGGATCATGCGATTACCTCAAGAGGATTTCTGTCAGACATTAAATATTCCAGCAGCCAAAAA
ATATGAAAGTCATGGCGGCCCAGGTATTGAAAAAATAATGCCGTACCTACTGGGTTCTAGGAATGCAGAGCAAGATAGAT
ACGACTTCATGAAGTCTCAGGTACTGTTTTGGTTATTAGCAGCTACAGATGGTCATGCAAAAAACTTCTCACTCTTCATT
GAGGCCGGTGCCGGGTATCGATTGACTCCCTTTTACGATATTTTGTCCATGTACCCCGTGATTGGTGGTAAGGGCTTAAA
TATTCGTGATGCTAAGTTGGCTATGGGACTTAAGGCTACTAAAGGTAAGAAGTATCAGGTTGCACAGATCTTCCCGCGGC
ATTTTATGCAAACTGCAAAAGCAGTCGGTTTTGACGTTGCCGCTATGTCCGCTATCATGCGCGAGATAGCCAGCTCCGTT
GATTTGGTGATGGAAAAAGTACAAGCGCAGTTACCCGATAGCTTCCCTGTTTATATTCGCGATGCAATACTTGATGGGCT
AAAAATAAGATCGAAGAGGCTGGTCGATAAGGAGGGTTGCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT154937 WP_001312331.1 NZ_CP051711:3301021-3301317 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT154937 NZ_CP068437:c4944289-4944008 [Shewanella sp. KX20019]
ATGAAGGTGACTAACTCAAAGCAGCTTAGCGCGTATCTCAAAGATGCACGATTGAACATGAAACTGTCGCAAAGCAAAGT
GGCACATAAGGTAGGCATTCGTCAGGATACGGTTTCAAGCTTTGAGCAAAACCCTGGTTCCACCAAATTAGAAACGCTAT
TTAAGATTTTATCAGCTTTAAATCTTGAGCTAGACATTAAGGTTAGAAATCCAGAGCTGGCGAGTAAGTTACTGGACGAT
AGAACCATGAGCTCTGACCGTGAATGGAAAGAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References