Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3876629..3877179 Replicon chromosome
Accession NZ_CP051706
Organism Escherichia coli strain SCU-124

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HHJ37_RS18740 Protein ID WP_001160966.1
Coordinates 3876629..3876943 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HHJ37_RS18745 Protein ID WP_000125566.1
Coordinates 3876946..3877179 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ37_RS18715 3872304..3873590 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HHJ37_RS18720 3873590..3874579 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HHJ37_RS18725 3874576..3875397 + 822 WP_001523934.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HHJ37_RS18730 3875400..3875777 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HHJ37_RS18735 3875784..3876632 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HHJ37_RS18740 3876629..3876943 - 315 WP_001160966.1 CcdB family protein Toxin
HHJ37_RS18745 3876946..3877179 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HHJ37_RS18750 3877265..3877744 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HHJ37_RS18755 3877936..3879798 - 1863 WP_001523933.1 glutathione-regulated potassium-efflux system protein KefC -
HHJ37_RS18760 3879791..3880321 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HHJ37_RS18765 3880429..3881760 - 1332 WP_001523932.1 MFS transporter -
HHJ37_RS18770 3881819..3882106 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T154910 WP_001160966.1 NZ_CP051706:c3876943-3876629 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T154910 NZ_CP068394:2231938-2232312 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT154910 WP_000125566.1 NZ_CP051706:c3877179-3876946 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT154910 NZ_CP068394:2231481-2231849 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References