Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 291037..291837 Replicon chromosome
Accession NZ_CP051706
Organism Escherichia coli strain SCU-124

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag HHJ37_RS01325 Protein ID WP_000342452.1
Coordinates 291310..291837 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag HHJ37_RS01320 Protein ID WP_001277107.1
Coordinates 291037..291303 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ37_RS01300 286696..287364 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
HHJ37_RS01305 287357..288415 + 1059 WP_001525854.1 permease-like cell division protein FtsX -
HHJ37_RS01310 288660..289514 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HHJ37_RS01315 289785..290888 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HHJ37_RS01320 291037..291303 + 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
HHJ37_RS01325 291310..291837 + 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
HHJ37_RS01330 291834..292217 - 384 WP_001525853.1 aspartate 1-decarboxylase autocleavage activator PanM -
HHJ37_RS01335 292640..293749 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HHJ37_RS01340 293797..294723 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HHJ37_RS01345 294720..295997 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
HHJ37_RS01350 295994..296761 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T154894 WP_000342452.1 NZ_CP051706:291310-291837 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T154894 NZ_CP068390:c318825-318397 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGATAGTACTCGACACAAATGTGTTGTCAGAACTGATGCGCCCTCAACCGGATACGGCGGTATTGCTGTGGATCGAAGC
GCAACCCGTCGAGGACCTCTACATCAGCGCTATGACCATGGCCGAGATTCTCCACGGTATCGCGCGCCTGCCCCACGGCA
AGCGCAAACAGACGCTGCATGCATCGGCGGTGGCCATGTTCGAGGAAGACTTCATCGACCGGATCGTGCCCTTCGGAACC
CAAGCGGCTCTTTACTACGCGCATCTGGTTTCGCATCGCGAAAGATCAGGCCAGCCGATCAATCTGGTGGATGCGCAAAT
TGCAGCGATATGCCGGGTCCACGACGCCGCGATCGCCACTCGTAACACCAAGGATTTCACCGACACCGGGCTGATCGTTA
TCAACCCCTGGCTGCCGCTCGAGGTCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT154894 WP_001277107.1 NZ_CP051706:291037-291303 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT154894 NZ_CP068390:c319067-318822 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGGCCAGCCTTACGATCCGTAATCTGGACGACCACATCAAAGAACGCCTGCGCATCGAGGCCGCTCACAATGGTCATTC
CATGGAAGAAGAAGCCCGATTGATTTTGCGCCGCGCATTGAGCAGGACAGCTGACAGCCAAGGCCTGGGCAGCCGTATCA
GGGCACGCTTTGCCGAGACCGGCGGAGTAGAGCTGGAGCTGCCTGAACGTACCGAACCGGCACGCTTGGTGGACCTGGGC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References