Detailed information of TA system TA154893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1778..2337
• Replicon: plasmid pSCU-125-2
• Accession: NZ_CP051702
• Organism: Escherichia coli strain SCU-125

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: HHJ38_RS25355
• Protein ID: WP_000421257.1
• Coordinates: 2062..2337 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: HHJ38_RS25350
• Protein ID: WP_001178087.1
• Coordinates: 1778..2062 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ38_RS25345	1..879	+	879	WP_000729439.1	incFII family plasmid replication initiator RepA	-
HHJ38_RS25350	1778..2062	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
HHJ38_RS25355	2062..2337	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ38_RS25360	2443..2724	+	282	WP_001105066.1	hypothetical protein	-
HHJ38_RS26020	2915..3181	-	267	WP_000627831.1	hypothetical protein	-
HHJ38_RS25365	3319..3921	-	603	WP_000517694.1	hypothetical protein	-
HHJ38_RS25370	4275..5621	+	1347	WP_001132037.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA5 / qacE / sul1 / aph(6)-Id / aph(3'')-Ib / sul2	-	1..93339	93339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T154893 WP_000421257.1 NZ_CP051702:2062-2337 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T154893 NZ_CP068390:311549-311731 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCCGTA
CAAACATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT154893 WP_001178087.1 NZ_CP051702:1778-2062 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT154893 NZ_CP068390:311761-312162 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCAGCGCAAGCCTTCGAGAATGTGAAAGAAGCCCTGTCGCTGCACTACGAAGGCCTGGTCGCCGATG
GTGAGCCGGTGCCCCAGGTGCATGAAGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGGGTGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGTTTTCTCGCGGCAGCGGCATTGCGCGAGTTGTCGGCGT
AG

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: U9Y625