Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 113259..113784 Replicon plasmid pSCU-125-1
Accession NZ_CP051701
Organism Escherichia coli strain SCU-125

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HHJ38_RS25310 Protein ID WP_001159871.1
Coordinates 113259..113564 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HHJ38_RS25315 Protein ID WP_000813634.1
Coordinates 113566..113784 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ38_RS25280 108525..109796 + 1272 WP_021549745.1 Y-family DNA polymerase -
HHJ38_RS25285 109801..110193 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
HHJ38_RS25290 110198..111169 - 972 WP_001103694.1 hypothetical protein -
HHJ38_RS25295 111398..112042 + 645 WP_000633911.1 AAA family ATPase -
HHJ38_RS25300 112036..112311 + 276 WP_000239529.1 hypothetical protein -
HHJ38_RS25305 112449..113258 - 810 WP_000016979.1 site-specific integrase -
HHJ38_RS25310 113259..113564 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HHJ38_RS25315 113566..113784 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HHJ38_RS25320 116106..116747 + 642 WP_021537822.1 hypothetical protein -
HHJ38_RS25325 116978..117367 + 390 WP_001282151.1 IS66 family insertion sequence hypothetical protein -
HHJ38_RS25330 117364..117711 + 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..120617 120617


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T154892 WP_001159871.1 NZ_CP051701:c113564-113259 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T154892 NZ_CP068389:c1871312-1870962 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAAATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT154892 WP_000813634.1 NZ_CP051701:c113784-113566 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT154892 NZ_CP068389:c1871598-1871317 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References