Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4210919..4211573 Replicon chromosome
Accession NZ_CP051700
Organism Escherichia coli strain SCU-125

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HHJ38_RS20655 Protein ID WP_000244781.1
Coordinates 4211166..4211573 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HHJ38_RS20650 Protein ID WP_000354046.1
Coordinates 4210919..4211185 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ38_RS20630 4207007..4208440 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
HHJ38_RS20635 4208485..4208796 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
HHJ38_RS20640 4208960..4209619 + 660 WP_000250274.1 hemolysin III family protein -
HHJ38_RS20645 4209696..4210676 - 981 WP_000886080.1 tRNA-modifying protein YgfZ -
HHJ38_RS20650 4210919..4211185 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HHJ38_RS20655 4211166..4211573 + 408 WP_000244781.1 protein YgfX Toxin
HHJ38_RS20660 4211613..4212134 - 522 WP_001055874.1 flavodoxin FldB -
HHJ38_RS20665 4212246..4213142 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HHJ38_RS20670 4213167..4213877 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HHJ38_RS20675 4213883..4215616 + 1734 WP_169063607.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T154889 WP_000244781.1 NZ_CP051700:4211166-4211573 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T154889 NZ_CP068387:c2986-2705 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT154889 WP_000354046.1 NZ_CP051700:4210919-4211185 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT154889 NZ_CP068387:c3227-2976 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References