Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2753927..2754424 Replicon chromosome
Accession NZ_CP051700
Organism Escherichia coli strain SCU-125

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HHJ38_RS13725 Protein ID WP_001173343.1
Coordinates 2753927..2754100 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HHJ38_RS13730 Protein ID WP_001312331.1
Coordinates 2754128..2754424 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ38_RS13715 2750597..2752054 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
HHJ38_RS13720 2752291..2753808 + 1518 WP_169063571.1 lysine--tRNA ligase -
HHJ38_RS13725 2753927..2754100 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HHJ38_RS13730 2754128..2754424 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HHJ38_RS13735 2754652..2754924 - 273 WP_000405647.1 N-acetyltransferase -
HHJ38_RS13740 2754936..2755166 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
HHJ38_RS13745 2755347..2756978 + 1632 WP_001216466.1 sensor histidine kinase -
HHJ38_RS13750 2756975..2757694 + 720 WP_000611282.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T154887 WP_001173343.1 NZ_CP051700:c2754100-2753927 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T154887 NZ_CP068386:c4213284-4212886 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAATTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT154887 WP_001312331.1 NZ_CP051700:c2754424-2754128 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT154887 NZ_CP068386:c4213511-4213284 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References