Detailed information of TA system TA154883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2359425..2360001
• Replicon: chromosome
• Accession: NZ_CP051700
• Organism: Escherichia coli strain SCU-125

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: HHJ38_RS11755
• Protein ID: WP_001297643.1
• Coordinates: 2359714..2360001 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HHJ38_RS11750
• Protein ID: WP_021538262.1
• Coordinates: 2359425..2359727 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ38_RS11735	2356021..2358171	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
HHJ38_RS11740	2358221..2358424	+	204	WP_000467859.1	YbdD/YjiX family protein	-
HHJ38_RS11745	2358435..2359391	+	957	WP_001297640.1	GTPase	-
HHJ38_RS11750	2359425..2359727	-	303	WP_021538262.1	BrnA antitoxin family protein	Antitoxin
HHJ38_RS11755	2359714..2360001	-	288	WP_001297643.1	BrnT family toxin	Toxin
HHJ38_RS11760	2360200..2360897	-	698	Protein_2293	IS1 family transposase	-
HHJ38_RS11765	2360954..2361049	+	96	Protein_2294	dienelactone hydrolase family protein	-
HHJ38_RS11770	2361195..2362427	+	1233	WP_169063640.1	multidrug efflux MFS transporter MdtM	-
HHJ38_RS11775	2362468..2363748	+	1281	WP_169063554.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2360200..2360409	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T154883 WP_001297643.1 NZ_CP051700:c2360001-2359714 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T154883 NZ_CP068386:c1438766-1438632 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
GTGACGGTGAAGATTTCGGACGGGTGTATTGTGCTAATGGCGGACGGTAACGAGGTGCAGGAGCTGCGCGAACAGCTTTA
TCAGGCTAAACGGGTGGTTAAGGGGGGTGAAGGATGTGCTGGTTTAGTGGACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11531.00 Da
• Isoelectric Point: 9.4651

>AT154883 WP_021538262.1 NZ_CP051700:c2359727-2359425 [Escherichia coli]
MSMVKHKRGTSSTLDVHHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT154883 NZ_CP068386:1438682-1439034 [Salmonella enterica subsp. enterica serovar Gallinarum/Pullorum]
CCTGATAAAGCTGTTCGCGCAGCTCCTGCACCTCGTTACCGTCCGCCATTAGCACAATACACCCGTCCGAAATCTTCACC
GTCACGCCGCTCCCCGGTCTCAAATCCCGCTTCTTCCTGGCAGTCGCCGCCGGGGATTTGTGAGTAACAGGTGGTCATGC
AGGTTTCGCTGTTGATGTGGCGGACGCTGGCGCCGGAGGTATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCC
GGATGCTGGCTGATTGTGGAGCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCCCCCTCTCCCCGGCCA
GTGATGAGCAACCGGCTACCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: A0A829GEV9