Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4270624..4271121 Replicon chromosome
Accession NZ_CP051698
Organism Escherichia coli strain SCU-152

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HHJ40_RS20365 Protein ID WP_001173343.1
Coordinates 4270624..4270797 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HHJ40_RS20370 Protein ID WP_000398619.1
Coordinates 4270825..4271121 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ40_RS20355 4267294..4268751 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
HHJ40_RS20360 4268988..4270505 + 1518 WP_001295074.1 lysine--tRNA ligase -
HHJ40_RS20365 4270624..4270797 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HHJ40_RS20370 4270825..4271121 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HHJ40_RS20375 4271348..4271620 - 273 WP_000405647.1 N-acetyltransferase -
HHJ40_RS20380 4271632..4271862 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
HHJ40_RS20385 4272043..4273674 + 1632 WP_001216486.1 sensor histidine kinase -
HHJ40_RS20390 4273671..4274390 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T154862 WP_001173343.1 NZ_CP051698:c4270797-4270624 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T154862 NZ_CP068287:30539-30853 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT154862 WP_000398619.1 NZ_CP051698:c4271121-4270825 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT154862 NZ_CP068287:30850-31269 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGTCAGATTTGGGGTATCAC
CTGCGCTATTTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References