154857

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3549735..3550572 Replicon chromosome
Accession NZ_CP051698
Organism Escherichia coli strain SCU-152

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag HHJ40_RS17020 Protein ID WP_000227784.1
Coordinates 3550030..3550572 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HHJ40_RS17015 Protein ID WP_001297137.1
Coordinates 3549735..3550046 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ40_RS16990 3544755..3545702 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
HHJ40_RS16995 3545724..3547715 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HHJ40_RS17000 3547705..3548319 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HHJ40_RS17005 3548319..3548648 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HHJ40_RS17010 3548660..3549550 + 891 WP_000971336.1 heme o synthase -
HHJ40_RS17015 3549735..3550046 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HHJ40_RS17020 3550030..3550572 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
HHJ40_RS17025 3550628..3551563 - 936 WP_001297127.1 sel1 repeat family protein -
HHJ40_RS17030 3551971..3553335 + 1365 WP_001000960.1 MFS transporter -
HHJ40_RS17035 3553463..3553954 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HHJ40_RS17040 3554122..3555033 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T154857 WP_000227784.1 NZ_CP051698:3550030-3550572 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T154857 NZ_CP068285:c2159904-2159644 [Ralstonia syzygii subsp. celebesensis]
CTGCGCGACAAGCGTGCCAAGGCGCGGATCGCCGCCCGGCTGGTCAGCGCATCGTTCGGGAATTTCGGCGACTGCAAGCC
GGTGGGTGAAGGCGTGAGCGAGATGCGGATCGATGTGGGCGCCGGGTATCGCGTCTACTACACGCGACAAGGTACTGTCG
TCTACATCCTGCTCACGGGCGGCAACAAGGCAACCCAAGCCCAGGACATCAAGCAAGCCATCCGGATGGCACGCGACCTG
AAGGAGTCAGGCCATGACTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT154857 WP_001297137.1 NZ_CP051698:3549735-3550046 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT154857 NZ_CP068285:c2159651-2159364 [Ralstonia syzygii subsp. celebesensis]
ATGACTGAAACCTACGCACCGTTTGACGCGGCGGCCTACCTCGATAGCGAAGAGACCATCGCCGAATTTCTGGCGGCCGC
GATGGAAGACCCGAATCCGGATGTCTTCCTGGCTGCCCTCGGTGCCGTTGCACGCGCCTGCGGCATTGCCAAGGTGGCTG
CGGACGCGGGCCTGGGGCGCGAAAGCCTGTACAAGGCCATCAGCCCCGGCGCGCACCCGCGCTACGAAACCATCCAGAAA
ATCATGCGCGCACTGGGCGTCAGGCTGAACGTGACGCCTGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References