Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3515656..3516274 Replicon chromosome
Accession NZ_CP051698
Organism Escherichia coli strain SCU-152

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HHJ40_RS16850 Protein ID WP_001291435.1
Coordinates 3516056..3516274 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HHJ40_RS16845 Protein ID WP_000344800.1
Coordinates 3515656..3516030 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ40_RS16835 3510745..3511938 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HHJ40_RS16840 3511961..3515110 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HHJ40_RS16845 3515656..3516030 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HHJ40_RS16850 3516056..3516274 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HHJ40_RS16855 3516446..3516997 + 552 WP_000102564.1 maltose O-acetyltransferase -
HHJ40_RS16860 3517113..3517583 + 471 WP_000136192.1 YlaC family protein -
HHJ40_RS16865 3517747..3519297 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HHJ40_RS16870 3519339..3519692 - 354 WP_000878141.1 DUF1428 family protein -
HHJ40_RS16880 3520071..3520382 + 312 WP_000409911.1 MGMT family protein -
HHJ40_RS16885 3520413..3520985 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T154856 WP_001291435.1 NZ_CP051698:3516056-3516274 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T154856 NZ_CP068285:c2159651-2159364 [Ralstonia syzygii subsp. celebesensis]
ATGACTGAAACCTACGCACCGTTTGACGCGGCGGCCTACCTCGATAGCGAAGAGACCATCGCCGAATTTCTGGCGGCCGC
GATGGAAGACCCGAATCCGGATGTCTTCCTGGCTGCCCTCGGTGCCGTTGCACGCGCCTGCGGCATTGCCAAGGTGGCTG
CGGACGCGGGCCTGGGGCGCGAAAGCCTGTACAAGGCCATCAGCCCCGGCGCGCACCCGCGCTACGAAACCATCCAGAAA
ATCATGCGCGCACTGGGCGTCAGGCTGAACGTGACGCCTGCCGCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT154856 WP_000344800.1 NZ_CP051698:3515656-3516030 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT154856 NZ_CP068285:c2159904-2159644 [Ralstonia syzygii subsp. celebesensis]
CTGCGCGACAAGCGTGCCAAGGCGCGGATCGCCGCCCGGCTGGTCAGCGCATCGTTCGGGAATTTCGGCGACTGCAAGCC
GGTGGGTGAAGGCGTGAGCGAGATGCGGATCGATGTGGGCGCCGGGTATCGCGTCTACTACACGCGACAAGGTACTGTCG
TCTACATCCTGCTCACGGGCGGCAACAAGGCAACCCAAGCCCAGGACATCAAGCAAGCCATCCGGATGGCACGCGACCTG
AAGGAGTCAGGCCATGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References