Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1387772..1388397 Replicon chromosome
Accession NZ_CP051698
Organism Escherichia coli strain SCU-152

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag HHJ40_RS06610 Protein ID WP_000911329.1
Coordinates 1387999..1388397 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HHJ40_RS06605 Protein ID WP_000450524.1
Coordinates 1387772..1387999 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ40_RS06580 1383574..1384044 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
HHJ40_RS06585 1384044..1384616 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HHJ40_RS06590 1384762..1385640 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HHJ40_RS06595 1385657..1386691 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HHJ40_RS06600 1386904..1387617 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HHJ40_RS06605 1387772..1387999 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HHJ40_RS06610 1387999..1388397 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
HHJ40_RS06615 1388544..1389407 + 864 WP_001267498.1 neutral zinc metallopeptidase -
HHJ40_RS06620 1389422..1391437 + 2016 WP_000829342.1 tRNA cytosine(34) acetyltransferase TmcA -
HHJ40_RS06625 1391511..1392209 + 699 WP_000679812.1 esterase -
HHJ40_RS06630 1392290..1392490 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T154845 WP_000911329.1 NZ_CP051698:1387999-1388397 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T154845 NZ_CP068283:c3292542-3292228 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT154845 WP_000450524.1 NZ_CP051698:1387772-1387999 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT154845 NZ_CP068283:c3292231-3291815 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References