Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 73692..74217 Replicon plasmid pSCU-313-1
Accession NZ_CP051695
Organism Escherichia coli strain SCU-313

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HHJ46_RS24855 Protein ID WP_001159868.1
Coordinates 73692..73997 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HHJ46_RS24860 Protein ID WP_000813634.1
Coordinates 73999..74217 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ46_RS24840 69602..70768 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HHJ46_RS24845 71356..72111 - 756 WP_000852146.1 replication initiation protein RepE -
HHJ46_RS24850 72885..73691 - 807 WP_000016982.1 site-specific integrase -
HHJ46_RS24855 73692..73997 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HHJ46_RS24860 73999..74217 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HHJ46_RS24865 74784..75296 + 513 WP_000151784.1 hypothetical protein -
HHJ46_RS24870 75330..76463 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
HHJ46_RS24875 76630..77403 - 774 WP_000905949.1 hypothetical protein -
HHJ46_RS24880 77416..77916 - 501 WP_000528931.1 hypothetical protein -
HHJ46_RS24885 78181..78411 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HHJ46_RS24890 78408..78822 + 415 Protein_99 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / aac(3)-IId / blaTEM-1B / sitABCD iutA / iucD / iucC / iucB / iucA 1..105394 105394


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T154831 WP_001159868.1 NZ_CP051695:c73997-73692 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T154831 NZ_CP068283:c1305161-1305054 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT154831 WP_000813634.1 NZ_CP051695:c74217-73999 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT154831 NZ_CP068283:1305208-1305274 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References