Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4614474..4614971 Replicon chromosome
Accession NZ_CP051694
Organism Escherichia coli strain SCU-313

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HHJ46_RS22230 Protein ID WP_001173343.1
Coordinates 4614474..4614647 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HHJ46_RS22235 Protein ID WP_000398619.1
Coordinates 4614675..4614971 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ46_RS22220 4611145..4612602 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
HHJ46_RS22225 4612839..4614356 + 1518 WP_000003806.1 lysine--tRNA ligase -
HHJ46_RS22230 4614474..4614647 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HHJ46_RS22235 4614675..4614971 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HHJ46_RS22240 4615200..4615472 - 273 WP_000405647.1 N-acetyltransferase -
HHJ46_RS22245 4615484..4615714 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
HHJ46_RS22250 4615895..4617526 + 1632 WP_001216477.1 sensor histidine kinase -
HHJ46_RS22255 4617523..4618242 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T154829 WP_001173343.1 NZ_CP051694:c4614647-4614474 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T154829 NZ_CP068283:c1304091-1303984 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT154829 WP_000398619.1 NZ_CP051694:c4614971-4614675 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT154829 NZ_CP068283:1304139-1304205 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References