Detailed information of TA system TA154784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 65984..66543
• Replicon: plasmid pSCU-387-1
• Accession: NZ_CP051689
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A376ZSB3
• Locus tag: HHJ48_RS26040
• Protein ID: WP_021515398.1
• Coordinates: 65984..66259 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HHJ48_RS26045
• Protein ID: WP_024187480.1
• Coordinates: 66259..66543 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS26025	63288..64064	-	777	WP_097403217.1	energy transducer TonB	-
HHJ48_RS26030	64072..64947	-	876	WP_123004305.1	ChaN family lipoprotein	-
HHJ48_RS26035	65224..65718	+	495	Protein_44	transposase	-
HHJ48_RS26040	65984..66259	-	276	WP_021515398.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ48_RS26045	66259..66543	-	285	WP_024187480.1	ribbon-helix-helix domain-containing protein	Antitoxin
HHJ48_RS26050	67090..67518	-	429	WP_097403215.1	Exc2 family lipoprotein	-
HHJ48_RS26055	67599..67697	-	99	Protein_48	entry exclusion protein 1	-
HHJ48_RS26060	67782..68171	+	390	WP_001282151.1	IS66 family insertion sequence hypothetical protein	-
HHJ48_RS26065	68168..68515	+	348	WP_021548229.1	IS66 family insertion sequence element accessory protein TnpB	-
HHJ48_RS26070	68565..70103	+	1539	WP_016245515.1	IS66 family transposase	-
HHJ48_RS26075	70184..70447	-	264	Protein_52	type II toxin-antitoxin system RelE/ParE family toxin	-
HHJ48_RS26080	70437..70700	-	264	WP_001089474.1	hypothetical protein	-
HHJ48_RS26895	70807..71067	-	261	WP_097403166.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroB / iroC / iroD / iroE / iroN / senB	1..139635	139635
	-	inside	IScluster/Tn	-	-	65452..70103	4651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10778.55 Da
• Isoelectric Point: 10.2344

>T154784 WP_021515398.1 NZ_CP051689:c66259-65984 [Escherichia coli]
MELKWTSKALSNLARLYDFLVLASKPAAARMVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T154784 NZ_CP068279:c3248589-3248338 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10470.89 Da
• Isoelectric Point: 5.6558

>AT154784 WP_024187480.1 NZ_CP051689:c66543-66259 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT154784 NZ_CP068279:c3248341-3248087 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A376ZSB3