154777

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3915056..3915639 Replicon chromosome
Accession NZ_CP051688
Organism Escherichia coli strain SCU-387

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag HHJ48_RS19075 Protein ID WP_000254749.1
Coordinates 3915056..3915391 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HHJ48_RS19080 Protein ID WP_000581937.1
Coordinates 3915391..3915639 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ48_RS19060 3910942..3912240 - 1299 WP_000036723.1 phosphopyruvate hydratase -
HHJ48_RS19065 3912328..3913965 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HHJ48_RS19070 3914193..3914984 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HHJ48_RS19075 3915056..3915391 - 336 WP_000254749.1 endoribonuclease MazF Toxin
HHJ48_RS19080 3915391..3915639 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HHJ48_RS19085 3915717..3917951 - 2235 WP_000226797.1 GTP diphosphokinase -
HHJ48_RS19090 3917999..3919300 - 1302 WP_169062297.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T154777 WP_000254749.1 NZ_CP051688:c3915391-3915056 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T154777 NZ_CP068279:c2459504-2459397 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT154777 WP_000581937.1 NZ_CP051688:c3915639-3915391 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT154777 NZ_CP068279:2459551-2459617 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References