Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 757082..757632 Replicon chromosome
Accession NZ_CP051688
Organism Escherichia coli strain SCU-387

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HHJ48_RS03600 Protein ID WP_001160966.1
Coordinates 757318..757632 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag HHJ48_RS03595 Protein ID WP_000125568.1
Coordinates 757082..757315 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ48_RS03570 752155..752442 + 288 WP_000203741.1 ferredoxin-like protein FixX -
HHJ48_RS03575 752501..753832 + 1332 WP_001183207.1 MFS transporter -
HHJ48_RS03580 753940..754470 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HHJ48_RS03585 754463..756325 + 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
HHJ48_RS03590 756517..756996 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HHJ48_RS03595 757082..757315 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HHJ48_RS03600 757318..757632 + 315 WP_001160966.1 CcdB family protein Toxin
HHJ48_RS03605 757629..758477 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HHJ48_RS03610 758484..758861 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HHJ48_RS03615 758864..759685 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HHJ48_RS03620 759682..760671 - 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HHJ48_RS03625 760671..761957 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T154761 WP_001160966.1 NZ_CP051688:757318-757632 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T154761 NZ_CP068278:c99646-99386 [Corynebacterium aquatimens]
GTGTTAGTCCGTTGGGAGCGGGACGCATGGGAAGAATATTGCCGGAGGCAAATTGAGGATCGGAGGATCCTCAAACGAAT
TAACACCCTGATCACTGACGTATTGCGGAACGGCAATGAGGGTATTGGTAAACCGGAACCGCTGAAGCACGACTTTTCGG
GGTACTGGTCACGCCGCATCAACAGCGAACACCGGCTGATTTACAAGATCGACGACGGCGGCATAACCATCGTTGCTTGC
CGCTACCACTACGGTGAATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT154761 WP_000125568.1 NZ_CP051688:757082-757315 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT154761 NZ_CP068278:c99903-99640 [Corynebacterium aquatimens]
GTGAGAATCATGTCCTACACGGAGTCCCGCGCCAAGTATGCAGACATGCTCACTTCAGTAGTGGATGACTGCGAAGAAGT
CATCATCACCCGCGCAGGCCACGAGCCCGTAGTTGTCGTCTCCCTGGCTGAGTACGAGTCCCTCAAGGAGACCGCCTACC
TGCTCCGCTCGCCCGCAAACGCACGCATGCTGAAAGAACGGATTGCCCGGGTGGAACGGGGGCAAGCGGAAGAGCACGAG
CTCATTGAGGATGACACGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References