Detailed information of TA system TA154754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 603110..603924
• Replicon: chromosome
• Accession: NZ_CP051688
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: HHJ48_RS02860
• Protein ID: WP_001054376.1
• Coordinates: 603667..603924 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: S1NWL7
• Locus tag: HHJ48_RS02855
• Protein ID: WP_001540600.1
• Coordinates: 603110..603655 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS02830	598801..600114	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
HHJ48_RS02835	600126..600404	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
HHJ48_RS02840	600401..601522	-	1122	WP_000010829.1	M42 family metallopeptidase	-
HHJ48_RS26805	601767..601883	-	117	Protein_551	VOC family protein	-
HHJ48_RS02845	601921..602139	-	219	Protein_552	hypothetical protein	-
HHJ48_RS02850	602308..603054	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
HHJ48_RS02855	603110..603655	-	546	WP_001540600.1	N-acetyltransferase	Antitoxin
HHJ48_RS02860	603667..603924	-	258	WP_001054376.1	hypothetical protein	Toxin
HHJ48_RS02865	604301..604546	-	246	Protein_556	GNAT family N-acetyltransferase	-
HHJ48_RS02870	604662..605902	+	1241	Protein_557	helicase YjhR	-
HHJ48_RS02875	606485..607465	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
HHJ48_RS02880	607530..608636	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T154754 WP_001054376.1 NZ_CP051688:c603924-603667 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T154754 NZ_CP068249:c2637192-2636884 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGCTCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19926.87 Da
• Isoelectric Point: 6.3277

>AT154754 WP_001540600.1 NZ_CP051688:c603655-603110 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFGKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT154754 NZ_CP068249:c2637466-2637194 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: S1NWL7