Detailed information of TA system TA154743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 7540157..7540792
• Replicon: chromosome
• Accession: NZ_CP051680
• Organism: Cohnella sp. MFER-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HH215_RS31655
• Protein ID: WP_169283531.1
• Coordinates: 7540442..7540792 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HH215_RS31650
• Protein ID: WP_169283530.1
• Coordinates: 7540157..7540438 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH215_RS31640	7537471..7538610	+	1140	WP_169283528.1	outer membrane lipoprotein carrier protein LolA	-
HH215_RS31645	7538772..7539959	+	1188	WP_169283529.1	alanine racemase	-
HH215_RS31650	7540157..7540438	+	282	WP_169283530.1	ribbon-helix-helix protein, CopG family	Antitoxin
HH215_RS31655	7540442..7540792	+	351	WP_169283531.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HH215_RS31660	7540870..7542105	+	1236	WP_169283532.1	aminopeptidase	-
HH215_RS31665	7542283..7543713	+	1431	WP_169283533.1	extracellular solute-binding protein	-
HH215_RS31670	7544037..7545026	+	990	WP_169283534.1	zinc-binding alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12932.00 Da
• Isoelectric Point: 5.6439

>T154743 WP_169283531.1 NZ_CP051680:7540442-7540792 [Cohnella sp. MFER-1]
MIVKRGDVFYADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIEAKIHGMERDSVILLEQI
RTIDKQRLTDKITHLDDETMRKVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T154743 NZ_CP068244:459834-460175 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10753.49 Da
• Isoelectric Point: 9.6917

>AT154743 WP_169283530.1 NZ_CP051680:7540157-7540438 [Cohnella sp. MFER-1]
VANLHNTKRIMISLPDHLLREVDFVVAKENTNRSEIIRQAMKHYLVDRKKRLIRDAMQRGYLEMAKINLHMASEAFHAEE
DAESILGRLVSGV

Nucleotide

• Length: 282 bp

>AT154743 NZ_CP068244:459604-459834 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG