Detailed information of TA system TA154730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4317645..4319334
• Replicon: chromosome
• Accession: NZ_CP051663
• Organism: Escherichia coli strain 1162invT2

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: HH200_RS21085
• Protein ID: WP_000365807.1
• Coordinates: 4318414..4319334 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: HH200_RS21080
• Protein ID: WP_001295795.1
• Coordinates: 4317645..4318427 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH200_RS21075 (4314263)	4314263..4317361	-	3099	WP_000673070.1	type I restriction endonuclease	-
HH200_RS21080 (4317645)	4317645..4318427	+	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
HH200_RS21085 (4318414)	4318414..4319334	+	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HH200_RS21090 (4319499)	4319499..4320275	-	777	WP_000171155.1	site-specific integrase	-
HH200_RS21095 (4320483)	4320483..4320818	-	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
HH200_RS21100 (4321045)	4321045..4321203	-	159	Protein_4123	integrase	-
HH200_RS21105 (4321252)	4321252..4321437	+	186	WP_000146240.1	hypothetical protein	-
HH200_RS21110 (4321652)	4321652..4322266	+	615	WP_001019923.1	YagU family protein	-
HH200_RS21115 (4322337)	4322337..4322503	+	167	Protein_4126	tail fiber assembly protein	-
HH200_RS21120 (4322515)	4322515..4322844	-	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
HH200_RS21125 (4323151)	4323151..4323861	-	711	WP_001295798.1	fimbrial chaperone EcpE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	4311395..4359550	48155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T154730 WP_000365807.1 NZ_CP051663:4318414-4319334 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

>T154730 NZ_CP068238:3429490-3430068 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAAGAACCGCCTCGACTTCCTCCGCGAAGCCGAACGCCTGAAAGATGTCCTGCGCTCCGCGCA
TACGTCTTCCGGTCGCCCGGAAAGCACCGCCGAACACAGTTGGCGGCTGTGCCTGATGGCGCTGGCCTTCGAGGATCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGATCTCGGCGAAGCGATCCACGGCGACATCCCG
GCCGTCGAGCAGGCCGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGTTGACTCGTCACCTCGACGC
GCCGCTGCGCGAACGCCTGCTCGCCCTGTGGGATGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGGCCCTGCGCGAGCTACTCGACGCCAAGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT154730 WP_001295795.1 NZ_CP051663:4317645-4318427 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT154730 NZ_CP068238:3428847-3429401 [Pseudomonas aeruginosa]
ATGACCGAGTCTCTTCCTTCTTCGTCCCTGGGCCCCGCCCTGCGCCGTTGGCGCCTGCTGCACCGGGTCAAGCAGGCGCA
CGCCGCCGAGCTGTTCGGCGTGGCCCAGTCGACCATCTCGCGCTGGGAATCCGGCGCCCAGGCGCTGCGGGCGGAGGAAC
GGACGCGCCTGGAAACCCTCCTCGGCGCCCGCCTGGAGACCGCCGCCGACCGGGCGCTGGCCCGCCTGGTCGATGGCAGC
CCGCAGCCGATGCATCTGATCTGCGACCTCACCCACCGCCTGCTGGCCTGCTCGCCGGCGCGCGCCGCGCAATTCGCCGT
CCCCCTCGGCGACCTGCTCGGCCGTTCGCTGTGGCCGTATTGCAGCGAGGAGATCCTCCGCCAGGAAGCCGCCCTCGACG
ACCTCGGCTGGCGCGAGCTGCTGGCGCCGCCGGCGCTGGAGTTCGCCAGCGGAACGAACGCCTCGACCATCGTGCCGATC
CGGCGCAGCCGCTGCCGCTGGACCCGCCTGACGCTCTCCGACGGCCGCGCGGTGCGGCTGGTGGAAACCCTCTGA

Structures

Toxin

• AlphaFold DB: A0A061KTE0