Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3940390..3940966 Replicon chromosome
Accession NZ_CP051663
Organism Escherichia coli strain 1162invT2

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag HH200_RS19260 Protein ID WP_001295743.1
Coordinates 3940390..3940677 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag HH200_RS19265 Protein ID WP_000063148.1
Coordinates 3940664..3940966 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH200_RS19245 (3936050) 3936050..3937354 - 1305 WP_000535012.1 restriction endonuclease subunit S -
HH200_RS19250 (3937344) 3937344..3938963 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
HH200_RS19255 (3939027) 3939027..3940193 - 1167 WP_000800831.1 restriction endonuclease -
HH200_RS19260 (3940390) 3940390..3940677 + 288 WP_001295743.1 BrnT family toxin Toxin
HH200_RS19265 (3940664) 3940664..3940966 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
HH200_RS19270 (3941000) 3941000..3941956 - 957 WP_001295745.1 GTPase -
HH200_RS19275 (3941967) 3941967..3942170 - 204 WP_000467859.1 YbdD/YjiX family protein -
HH200_RS19280 (3942220) 3942220..3944370 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T154726 WP_001295743.1 NZ_CP051663:3940390-3940677 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T154726 NZ_CP068237:c5022617-5021700 [Klebsiella quasipneumoniae]
ATGGATATACAGGCGCCCTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCCGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACGGCAATCAATCTCTTTATCCGTGATTTCCCACGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCGATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCGGGTATGAGCGCTGTATTACAAGCTAATCGTTCCGATGAGATGCGTGTCATTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTCTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTAGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCCGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGTCAGCAT
CCCAGAGATTTTTATGATGTGAAATTACTGCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCCTA
TTTACTGAGCCATAATCGGCCTCTGGCCGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCCTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGATGCGTTAAAATCC
TGTTTCACGCAACAGGATGTCGATTTTTTGCTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCGGAGTCATTAG
CAAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT154726 WP_000063148.1 NZ_CP051663:3940664-3940966 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT154726 NZ_CP068237:c5023381-5022623 [Klebsiella quasipneumoniae]
ATGCCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAATCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACAAGCAAAGTGGCTGGTTAACAAAACTAGGCAGCGGCGTCTATGCGCGTGCGG
GACGCAAGCCGGAGTGGAGCGATGCATTAGCGTGTCTGCAAAATCAGCTAGCCGCTCCGGTGTATGTCGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTTCAGCTTAAACAAAACCAGTGCTGGCTGAGTATTGAAAATAAAACGCT
GCTGCCAAAATGGTTCAGGGAATTCCCGGGTGTGGAATGGATAGTCATATCGGGGCAAAAACTGCCGGTGCTGGATGATA
AATACCGTGTAACGCTTGATGTAAAAGGGAAAAAACTCACCGGCAGTGCGCCGGAGCTGGCTGCTTACGAGTTGCTGAGC
GCCATACCGGGGACAATCTCATTTGACCATGCTGCGGAGCTTTTTCAGGGGCTGGTTAACCTAAATCCTCATAAAGTGGA
GTATCTGCTTTCTGTCAGCCAGTCCGTGCAGACCAAGCGTTTGTATCTCTTTTTCGCCAGTTTTTACGAGCATGCCTGGC
TCAGACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGTAAGCGGCAAATCGTCGTAAATGGCAAGTTCAATGCTCAA
TACCAAATCACTGTGCCTGAACGATTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References