Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3858072..3858907 Replicon chromosome
Accession NZ_CP051663
Organism Escherichia coli strain 1162invT2

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag HH200_RS18865 Protein ID WP_000854759.1
Coordinates 3858530..3858907 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag HH200_RS18860 Protein ID WP_001295723.1
Coordinates 3858072..3858440 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH200_RS18830 (3854120) 3854120..3854593 + 474 WP_001350782.1 antirestriction protein -
HH200_RS18835 (3854685) 3854685..3855110 + 426 WP_000422741.1 transposase -
HH200_RS18840 (3855107) 3855107..3855457 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
HH200_RS18845 (3855488) 3855488..3857101 + 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
HH200_RS18850 (3857149) 3857149..3857625 + 477 WP_001186775.1 RadC family protein -
HH200_RS18855 (3857688) 3857688..3857909 + 222 WP_000692345.1 DUF987 domain-containing protein -
HH200_RS18860 (3858072) 3858072..3858440 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HH200_RS18865 (3858530) 3858530..3858907 + 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
HH200_RS18870 (3858904) 3858904..3859392 + 489 WP_000761690.1 DUF5983 family protein -
HH200_RS18875 (3859409) 3859409..3859585 + 177 WP_000839286.1 DUF957 domain-containing protein -
HH200_RS18880 (3859691) 3859691..3859840 + 150 Protein_3690 hypothetical protein -
HH200_RS18885 (3860207) 3860207..3860383 + 177 Protein_3691 helix-turn-helix domain-containing protein -
HH200_RS18890 (3861174) 3861174..3862796 + 1623 WP_001295726.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T154725 WP_000854759.1 NZ_CP051663:3858530-3858907 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T154725 NZ_CP068237:4119647-4119865 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCGGAAC
TGACTATGAATAAGCTGTACGATAAGATCCCGACATCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT154725 WP_001295723.1 NZ_CP051663:3858072-3858440 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT154725 NZ_CP068237:4119246-4119620 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGTTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTCATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References