Detailed information of TA system TA154718

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2310052..2310886
• Replicon: chromosome
• Accession: NZ_CP051663
• Organism: Escherichia coli strain 1162invT2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: HH200_RS11440
• Protein ID: WP_000854690.1
• Coordinates: 2310509..2310886 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: HH200_RS11435
• Protein ID: WP_001305076.1
• Coordinates: 2310052..2310420 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH200_RS11395 (2305134)	2305134..2306261	+	1128	Protein_2237	hypothetical protein	-
HH200_RS11400 (2306337)	2306337..2306792	+	456	WP_000581502.1	IrmA family protein	-
HH200_RS11405 (2306871)	2306871..2307104	+	234	WP_000902034.1	DUF905 family protein	-
HH200_RS11410 (2307205)	2307205..2308023	+	819	WP_001234620.1	DUF932 domain-containing protein	-
HH200_RS11415 (2308078)	2308078..2308563	+	486	WP_000849565.1	antirestriction protein	-
HH200_RS11420 (2308579)	2308579..2309055	+	477	WP_001186726.1	RadC family protein	-
HH200_RS11425 (2309118)	2309118..2309339	+	222	WP_000692329.1	DUF987 domain-containing protein	-
HH200_RS11430 (2309358)	2309358..2310002	+	645	WP_000094916.1	hypothetical protein	-
HH200_RS11435 (2310052)	2310052..2310420	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HH200_RS11440 (2310509)	2310509..2310886	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
HH200_RS11445 (2310883)	2310883..2311371	+	489	WP_000761699.1	DUF5983 family protein	-
HH200_RS11450 (2311388)	2311388..2311585	+	198	WP_000839293.1	DUF957 domain-containing protein	-
HH200_RS11455 (2311670)	2311670..2312515	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
HH200_RS11460 (2312584)	2312584..2312979	+	396	WP_000208384.1	DUF6088 family protein	-
HH200_RS11465 (2312972)	2312972..2313905	+	934	Protein_2251	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HH200_RS11470 (2314322)	2314322..2314492	+	171	Protein_2252	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / ugd / kpsT	2265774..2332094	66320
	-	flank	IS/Tn	-	-	2314337..2314492	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T154718 WP_000854690.1 NZ_CP051663:2310509-2310886 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T154718 NZ_CP068235:c36902-36486 [Leclercia adecarboxylata]
GTGAACAAAACCTATATGCTGGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGTTGCGCAATCAGCGCATCGTGATCTCGGCCATTACTTACTCCGAGATACGCTTCGGCGCCACCGGTC
CGAAGGCCTCCCCGCGCCACACTGAGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACAGCGCT
GCGGTTGATGCGACTACAGAAATCAAGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATTGCCGCCGGCGCCGTGCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGCCTGATGCTGG
AAGACTGGGCCCGCTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT154718 WP_001305076.1 NZ_CP051663:2310052-2310420 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT154718 NZ_CP068235:c37129-36899 [Leclercia adecarboxylata]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ATAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCACCGACGAAGGACGATTTGACCTGTGA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8