Detailed information of TA system TA154715

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1231689..1232520
• Replicon: chromosome
• Accession: NZ_CP051663
• Organism: Escherichia coli strain 1162invT2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A066T988
• Locus tag: HH200_RS06465
• Protein ID: WP_000854815.1
• Coordinates: 1232146..1232520 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HH200_RS06460
• Protein ID: WP_234976396.1
• Coordinates: 1231689..1232057 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH200_RS06415 (1226782)	1226782..1227528	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
HH200_RS06420 (1227611)	1227611..1227961	+	351	Protein_1265	hypothetical protein	-
HH200_RS06425 (1227977)	1227977..1228387	+	411	WP_000846703.1	hypothetical protein	-
HH200_RS06430 (1228608)	1228608..1229426	+	819	WP_001542275.1	DUF932 domain-containing protein	-
HH200_RS06435 (1229426)	1229426..1229671	+	246	WP_001164966.1	hypothetical protein	-
HH200_RS06440 (1229764)	1229764..1230237	+	474	WP_234976394.1	antirestriction protein	-
HH200_RS06445 (1230252)	1230252..1230728	+	477	WP_234976395.1	RadC family protein	-
HH200_RS06450 (1230791)	1230791..1231012	+	222	WP_000692345.1	DUF987 domain-containing protein	-
HH200_RS06455 (1231031)	1231031..1231673	+	643	Protein_1272	antitoxin of toxin-antitoxin stability system	-
HH200_RS06460 (1231689)	1231689..1232057	+	369	WP_234976396.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HH200_RS06465 (1232146)	1232146..1232520	+	375	WP_000854815.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
HH200_RS06470 (1232517)	1232517..1232711	+	195	WP_000988600.1	DUF5983 family protein	-
HH200_RS06475 (1232757)	1232757..1232837	+	81	Protein_1276	hypothetical protein	-
HH200_RS06480 (1233126)	1233126..1233206	-	81	WP_023441679.1	hypothetical protein	-
HH200_RS06485 (1233185)	1233185..1233508	+	324	WP_225469267.1	EutP/PduV family microcompartment system protein	-
HH200_RS06490 (1233609)	1233609..1233938	-	330	WP_000450409.1	DUF496 family protein	-
HH200_RS06495 (1234110)	1234110..1235168	-	1059	WP_001200889.1	FUSC family protein	-
HH200_RS06500 (1235366)	1235366..1235839	-	474	WP_001105368.1	DNA gyrase inhibitor SbmC	-
HH200_RS06505 (1235958)	1235958..1237124	-	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13829.83 Da
• Isoelectric Point: 7.1326

>T154715 WP_000854815.1 NZ_CP051663:1232146-1232520 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T154715 NZ_CP068234:c2328456-2327149 [Leclercia adecarboxylata]
ATGCGCGCTCAACGTCTGACTGTTGCTATGAATGGCGAAGTGGTGGGTACGCTGCATCGTGATGGCAGCGGCGCGATGTC
ATTTCAGTATGCGCCCGAGTGGCTGGCAGAGCCTGGCGCACGTGCCATTTCGCTCTCTTTGCCGCTCCAGCACGGGAGGA
TCCGCGGAAAGCAGGTCTTCAATTTTTTCAGCAACCTGTTGCCAGACTCAGAGGCGATAATCGCCCGCATGCAGGCCCGT
TTTCAGGTTGAGACGGCACATCCCTTTGATCTGCTCGCCAGTGTCGGACGAGACTGCGTGGGTGCCATTCAGCTCTATCC
ACCGGGGCTGGAGATCCCATCGGTGATGGATATCGTCGCCGAACCGCTGGATGACGAGCAGATTGCTTCCTTGCTGGAGG
GTTACCAGATGGCCCCGTTAGGGATGAGCGAAGAGGGGGCTGATTTTCGCATTTCGCTGGCCGGGGCGCAGGAGAAAACG
GCGTTGCTATGGTATCAGGATCGCTGGCAGCGGCCTCAGGGTAGTACACCAACCAGTCATATATTCAAATTGCCGATCGG
CAGAATAGAGCAGAACAATATCGATCTCAGCGAGAGCTGTGAAAACGAGTGGCTCTGCTTACGGATCGCAAAAGCCTTTG
GATTTCCGGTAGCGCATGCCGAAATGGCCACCTTTAGTCGCAAAAAAGTGCTGATTATTGAACGCTTCGATCGCCGTTGG
GCCAGCAGCGGGTGGCTGATGCGCCTGCCGCAGGAAGATTTTTGTCAGGCGCTGGGCATCGCTCCGGCACTGAAGTATGA
GTCCCACGGCGGGCCGGGCATCGCCGATGCCATGAAACTGCTGCTCGGTTCCCGCCTGGCAGCGCAGGATCGGGAGATGT
TCTTCAAAGCGCAGATCCTGTTCTGGATGCTGGCCGCGATTGATGGTCACGGTAAGAATTTCAGCCTGTTTATCGAAGCG
GACTCTTCGTTTCGCATGGCCCCGTTATACGATGTGATTTCGGCCTTTCCGCTGTTTGAGGCAGGGAGTATCCCGGCCAA
AAAAGCGAAGATGGCCATGGCATTACAGGGCAAAAACCGCCAGTACCATTTCGCGATGATCCAGCCGCGTCACTTTATCA
GTACCGCTGCGCACGCAGGCTTTTCGCAGGAGATCGCCCGGAGGCTGATGAAAGAGATGGCCGACCGCACGGATGAGGCG
ATCGCCACCGTCAGGGCGGAATTGCCAGCGGATTTTCCGGAACAGATAAGCGAGGCGATTTTCCGCGGTCTGGTAAGCCA
GGCGGCGAGAATAAGCCGCTTTGATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13827.70 Da
• Isoelectric Point: 6.7391

>AT154715 WP_234976396.1 NZ_CP051663:1231689-1232057 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHTDRAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT154715 NZ_CP068234:c2328711-2328460 [Leclercia adecarboxylata]
ATGAAAGTGACCTCGGCAGGCGCACTGGCGCAGGCTGTCAAAGATCAGCGTAAGGTCAATAAGTTAACGCAATCAGAGAC
CGCTGAACAGGTTGGCATTAAACAAACCACGGTATCGGATTTCGAGAACAAACCTGAATCCACCAAGCTGGAAACCCTGT
TCAAAATTCTCTCGGCGCTGGATCTGGAACTTCACGTGGTGAAAAGAGGCTCGACGCTGCCCGATCATGCCAGCTGGACA
AAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A066T988