Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 128951..129476 Replicon plasmid pEco1162T7a
Accession NZ_CP051662
Organism Escherichia coli strain 1162T7

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HH201_RS25475 Protein ID WP_001159868.1
Coordinates 128951..129256 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HH201_RS25480 Protein ID WP_000813634.1
Coordinates 129258..129476 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH201_RS25460 (HH201_25230) 124861..126027 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HH201_RS25465 (HH201_25235) 126615..127370 - 756 WP_000852146.1 replication initiation protein RepE -
HH201_RS25470 (HH201_25240) 128144..128950 - 807 WP_000016982.1 site-specific integrase -
HH201_RS25475 (HH201_25245) 128951..129256 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HH201_RS25480 (HH201_25250) 129258..129476 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HH201_RS25485 (HH201_25255) 130184..131179 + 996 WP_000246636.1 hypothetical protein -
HH201_RS25490 (HH201_25260) 131222..132115 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catB3 / blaOXA-1 / aac(6')-Ib-cr senB 1..148312 148312
- flank IS/Tn - - 132252..132629 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T154700 WP_001159868.1 NZ_CP051662:c129256-128951 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T154700 NZ_CP068228:4050425-4051726 [Shewanella algae]
ATGCAAAAGCTAATAGCATACATGAATGGTGAACTTGTTGGCACCTTGGCGAAGCATAAGAACGGCGCTCACACTTTTCA
ATATGATAAGGACTGGATAACCAATGCAATGGCTCGCCCACTGTCATTGTCTTTGAGGCTTCAATTACCACCTATTACTT
CCGATGCCGTTATCAACTATTTTGACAACCTCTTACCAGACAGCCCACAAGTCAGAGACAGAATCGTTGCAAGGTACAAA
GCCTCATCCAAACAACCATTTGATCTTTTGAAAGAAATTGGAAAGGATAGTGTTGGTGCTATTGCACTATTTCCTCCCGA
ACACCCCTATAAAAAAGAGCCACTCAGCTACGAAATATTGGATAACAAGAAACTAGAAGCCGTTCTTTCTGCATACAAGT
CTGATATTCCCCTCGGAATGCTCAAAGAGGAAGAAGACTTTAGGATCTCAGTGGCAGGTGCTCAAGAAAAAACCGCACTC
TTATTCATTGATGGACAATGGTGTATACCGAAAAGAAACACGCCAACCACTCACATTATCAAGCTGCCAATAGGCGAAAT
ACCACAAGCTAGCGCGACACTGGATCTCAAAGATAGCGTAGAAAATGAATATCTTTGCATTGAGCTAGCTAAAGCGCTGG
GATTCGCAGTTCCCAGCGTCAATATAATCAAAACAGACAACCTTAAAGCTTTAGCTGTAGAACGATTTGATCGACGCTGG
ACAAAAGACAGAGCAGGCTTGCTACGCTTACCTCAAGAAGATATTTGCCAAGTGTTTGGTATGCCATCATCCATCAAATA
TGAATCTCAAGGTGGTCCAGGCATAGAACAGATCATGAAGTTATTAATGGGCTCAAGTAATGCACTTGAAGATCGATATA
GCTTCATGAGATTCCAGGTATTTCAATGGATCATTGGGGCTACGGATGGACATGCTAAAAACTTTTCTATCTATATTGAT
AGAGGAGGTAGCTATCGACTCACTCCATTCTACGACATTCTGTCGGCCTACCCATTACTGGGAGGAAAAGGACTCAATAT
TAGACAACTTAAGCTCGCAATGGGATTAAAAGCCACAAAAGGCAAGAAGTACGAAATCAATAAAATCCTGCCTCGTCACT
TTTTAGATACTGCTAAAGCTGTGAATTTTAGTCAGGATAAGATGCAAAGAATAATGGATGAAATGAAAGGTGCGTTACCT
AAGGCAATCTCGCAAGTAAGGGCGAATCTACCAGAGGGCTTTCCAGAGCATATCTCCAACTCAATTTTTGAGAATGCCAA
GAAAATAATTAAAAAAATCTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT154700 WP_000813634.1 NZ_CP051662:c129476-129258 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT154700 NZ_CP068228:4050177-4050425 [Shewanella algae]
ATGATATATAGTCCAAAGCAACTCGCAGACATGATGCTACTGATACGCCAAAAGAATGGCTGGACTCAATCAGAGTTGGC
GAAAAAAGTCGGTATAAAGCAAGCGACAATCTCGAACTTCGAGAACAACCCCAACAAAACAACGCTTTCTACCTTCTTTA
AGCTTGTACAAGCTATGGATCTAACACTGAGTATCCAAGAAAAAACACAGCTGACTTCGCCAAGTGAAAATGATGATGAG
AGTTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References