Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3588370..3588890 Replicon chromosome
Accession NZ_CP051661
Organism Escherichia coli strain 1162T7

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag HH201_RS17405 Protein ID WP_001160964.1
Coordinates 3588606..3588890 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HH201_RS17400 Protein ID WP_000125566.1
Coordinates 3588370..3588603 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH201_RS17375 (3583443) 3583443..3583730 + 288 WP_000203744.1 ferredoxin-like protein FixX -
HH201_RS17380 (3583789) 3583789..3585120 + 1332 WP_001183209.1 MFS transporter -
HH201_RS17385 (3585228) 3585228..3585758 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HH201_RS17390 (3585751) 3585751..3587613 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
HH201_RS17395 (3587805) 3587805..3588284 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HH201_RS17400 (3588370) 3588370..3588603 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HH201_RS17405 (3588606) 3588606..3588890 + 285 WP_001160964.1 CcdB family protein Toxin
HH201_RS17410 (3588917) 3588917..3589765 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HH201_RS17415 (3589772) 3589772..3590149 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HH201_RS17420 (3590152) 3590152..3590973 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HH201_RS17425 (3590970) 3590970..3591959 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HH201_RS17430 (3591959) 3591959..3593245 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T154692 WP_001160964.1 NZ_CP051661:3588606-3588890 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T154692 NZ_CP068226:c846651-845356 [Shewanella algae]
ATGCAAAAGCTAATAGCATACATGAATGGTGAACTTGTTGGCACCTTGGCGAAGCATAAGAACGGCGCTCACACTTTTCA
ATATGATAAGGACTGGATAACCAATGCAATGGCTCGCCCACTGTCATTGTCTTTGAGGCTTCAATTACCGACTATCACTT
CCGATGCCGTTATCAACTATTTTGACAACCTCTTACCAGACAGCCCACAAGTCAGAGATAGGATCGTTGCAAGGTACAAA
GCCTCGTCCAAACAACCATTTGATCTCTTGAAAGAAATTGGAAAGGATAGTGTTGGTGCTATTGCACTATTTCCTCCCGA
ACACCCCTATAAAAAAGAGCCACTCAGCTACGAAATATTGGATAACAAGAAACTAGAAGCCGTTCTTTCTGCATACAAGT
CTGATATTCCCCTCGGGATGCTCAAAGAGGAAGAAGACTTTAGGATCTCAGTGGCAGGTGCTCAAGAAAAAACCGCACTC
TTATTCATTGATGGACAATGGTGTATACCGAAAGGAAACACCCCAACCACTCACATTATCAAGCTGCCAATAGGCGAAAT
ACCACAAGCTAGCGCGACACTGGATCTCAAAGATAGCGTAGAAAATGAATATCTTTGCATTGAGCTAGCTAAAGCGCTGG
GATTCGCAGTTCCCAGCGTCAATATAATCAAAACAGACAACCTTAAAGCTTTAGCTGTAGAACGATTTGATCGACGCTGG
ACAAAAGACAGAGCAGGCTTGCTACGCTTACCTCAAGAAGATATTTGCCAGGTGTTTGGTATGCCATCATCCATCAAATA
TGAATCTCAAGGTGGTCCAGGCATAGAACAGATCATGAAGTTATTAATGGGCTCAAGTAATGCACTTGAAGATCGATATA
ACTTCATGAGATTCCAGGTATTTCAATGGATCATTGGGGCTACGGATGGACATGCTAAAAACTTTTCTATCTATATTAAT
AAAGGAGGTAGCTATCGACTCACTCCATTCTACGACATTCTTTCAGCCTACCCATTACTGGGAGGACTCAATATTCGACA
ACTTAAGCTCGCTATGGGATTAAAAGCCACAAAAGGCAAGAAGTATGAAATCAATAGAATCCTGCCTCGTCACTTTTTAG
ATACTGCGAAAGCTGTGAATTTTAGTCAGGATAAGATGCAAAGAATAATGGATGAAATGAAAGGTGCGTTACCTGAGGCA
ATCTCGCAAGTAAGGGCGAATCTACCCGAGGGCTTTCCAGAGAATATCTCCAACTCTATTTTTGAGAATGCCAAGAAAAC
AATTAAAAAAATCTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT154692 WP_000125566.1 NZ_CP051661:3588370-3588603 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT154692 NZ_CP068226:c846899-846651 [Shewanella algae]
ATGATATATAGTCCAAAGCAACTCGCAGATATGATGCTACTGATACGCCAAAAGAATGGCTGGACTCAGTCAGAGTTGGC
GAAAAAAGTCGGTATAAAGCAAGCAACAATCTCGAACTTCGAGAACAACCCCAACAAAACAACGCTTTCTACCTTCTTTA
AGCTTGTACAAGCTATGGATCTAACACTGAGTATCCAAGAAAAAACACAGCTGACTTCGCCAAGTGAAAATGATGATGAG
AGTTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References