Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3394769..3395604 Replicon chromosome
Accession NZ_CP051661
Organism Escherichia coli strain 1162T7

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag HH201_RS16470 Protein ID WP_000854759.1
Coordinates 3395227..3395604 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag HH201_RS16465 Protein ID WP_001295723.1
Coordinates 3394769..3395137 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH201_RS16435 (3390817) 3390817..3391290 + 474 WP_001350782.1 antirestriction protein -
HH201_RS16440 (3391382) 3391382..3391807 + 426 WP_000422741.1 transposase -
HH201_RS16445 (3391804) 3391804..3392154 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
HH201_RS16450 (3392185) 3392185..3393798 + 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
HH201_RS16455 (3393846) 3393846..3394322 + 477 WP_001186775.1 RadC family protein -
HH201_RS16460 (3394385) 3394385..3394606 + 222 WP_000692345.1 DUF987 domain-containing protein -
HH201_RS16465 (3394769) 3394769..3395137 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HH201_RS16470 (3395227) 3395227..3395604 + 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
HH201_RS16475 (3395601) 3395601..3396089 + 489 WP_000761690.1 DUF5983 family protein -
HH201_RS16480 (3396106) 3396106..3396282 + 177 WP_000839286.1 DUF957 domain-containing protein -
HH201_RS16485 (3396388) 3396388..3396537 + 150 Protein_3219 hypothetical protein -
HH201_RS16490 (3396904) 3396904..3397080 + 177 Protein_3220 helix-turn-helix domain-containing protein -
HH201_RS16495 (3397871) 3397871..3399493 + 1623 WP_001295726.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T154690 WP_000854759.1 NZ_CP051661:3395227-3395604 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T154690 NZ_CP068219:236816-236992 [Staphylococcus pasteuri]
ATGACACAAAATAAAGATTCTAAAACTAATTATCATGAAGAAGAAAATGCTATGGTAACTGATTTAGATGATTTAAAAGA
TTTAGGTAAAGAAATGGAACAAATTTCTGAGGAAAATGATGAAGATAAATTAAACCAATCACATGATTCAGATGTTCGTT
CTGATTTAGAAGAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT154690 WP_001295723.1 NZ_CP051661:3394769-3395137 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT154690 NZ_CP068219:236189-236788 [Staphylococcus pasteuri]
ATGGCAATGAACTTTAAAGTATTCGAAGATAAACAAAGAGTAGCTGAATATACAGCAGACATTTTGAGAAAACAGTTTAA
CAACAATCCTATGACTATCGCTGGTGTTCACTTAACTAAAGACCATGCACCTGTATTAGATGAACTTAAAAAGAACGTTG
AGTTACATGCAGTTGATTTTAGTCAAATTAACATTTTAGATTATGATGAAAATAAATCATACTATGAAGCTTTAGGCGTA
CCTCAAAGCCAAATTTATTCATTATCTTATAATCAAGATGCTAATGAATTTATTTCTGATAAAATTAAAACAAAAGAAAA
TAAAGGTAAATTAGTGTTACAAGTAGTTTCTATTGATGAGAATGGCAACCTTGATGTAAGCGTAAGACAAGGATTATTAG
AAGCGCGTGAAATTTTCTTAGTTGTTACTGGTAGCAATAAACGCGAAGTAGTTGAAAAATTATATAATGAAAATGGTAAG
TCTAATTATGAACCAGCAGATTTAAAAGCTCATCGCATGGTTAACGTTATTTTAGATAAAGAAGCAGCGGCTGGATTACC
TGAAGATGTTAAAGAATACTTCACTGCTCGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References