Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3192413..3192910 Replicon chromosome
Accession NZ_CP051661
Organism Escherichia coli strain 1162T7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HH201_RS15430 Protein ID WP_001173343.1
Coordinates 3192737..3192910 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HH201_RS15425 Protein ID WP_001312331.1
Coordinates 3192413..3192709 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH201_RS15415 (3187770) 3187770..3191699 + 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -
HH201_RS15420 (3191913) 3191913..3192185 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
HH201_RS15425 (3192413) 3192413..3192709 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HH201_RS15430 (3192737) 3192737..3192910 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HH201_RS15435 (3193029) 3193029..3194546 - 1518 WP_000003806.1 lysine--tRNA ligase -
HH201_RS15440 (3194783) 3194783..3196240 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3176822..3201764 24942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T154688 WP_001173343.1 NZ_CP051661:3192737-3192910 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T154688 NZ_CP068219:88112-88318 [Staphylococcus pasteuri]
GTGCAACAAAAAATCGTTTATACTTCAGGAATCAAAACGACTGAACAAGCTAACAAAATGGAAACAATATTAAAGGAAAT
GATAGGTGTATATGATGCAAATGTAGATTTAAGCAATGGTCACATTTCAGTTGAATTTGAGACACCTGCTAATTTAAATA
GTATCGAAAAAGAAATTTATGATTCTGGATTTAAAGTGCTTTATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT154688 WP_001312331.1 NZ_CP051661:3192413-3192709 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT154688 NZ_CP068219:87808-88101 [Staphylococcus pasteuri]
GTGACAGAACAAGATCATGCACATCACTCAGAACAAATTAAAACAAATTTGAAATCGAGATTAAATAGAATTGAAGGACA
AGTTCGTGCTATCAACCGAATGATAGAAGAAGATGTTTATTGTGATGATGTTTTAACACAAATTAGAGCAACACGTTCAG
CTTTAAATAGTGTAGCTACTAAATTATTAGAACAACATATGAAGAGTTGTATAACTGAAAAAGTCAATAATGGTCATCAA
GAAGAAGCCATGGAAGAGTTACTTGTAACTTTTCAAAAATTAATGAAAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References