Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2377000..2377800 Replicon chromosome
Accession NZ_CP051661
Organism Escherichia coli strain 1162T7

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag HH201_RS11695 Protein ID WP_000342452.1
Coordinates 2377000..2377527 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag HH201_RS11700 Protein ID WP_001277107.1
Coordinates 2377534..2377800 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH201_RS11670 (2372076) 2372076..2372843 - 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HH201_RS11675 (2372840) 2372840..2374117 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
HH201_RS11680 (2374114) 2374114..2375040 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HH201_RS11685 (2375088) 2375088..2376197 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HH201_RS11690 (2376620) 2376620..2377003 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
HH201_RS11695 (2377000) 2377000..2377527 - 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
HH201_RS11700 (2377534) 2377534..2377800 - 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
HH201_RS11705 (2377949) 2377949..2379052 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HH201_RS11710 (2379323) 2379323..2380177 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HH201_RS11715 (2380422) 2380422..2381480 - 1059 WP_001042013.1 permease-like cell division protein FtsX -
HH201_RS11720 (2381473) 2381473..2382141 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T154686 WP_000342452.1 NZ_CP051661:c2377527-2377000 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T154686 NZ_CP068216:c815259-815053 [Staphylococcus pasteuri]
GTGCAACAAAAAATCGTTTATACTTCAGGAATCAAAACGACTGAACAAGCTAACAAAATGGAAACAATATTAAAGGAAAT
GATAGGTGTATATGATGCAAATGTAGATTTAAGCAATGGTCACATTTCAGTTGAATTTGAGACACCTGCTAATTTAAATA
GTATAGAAAAAGAAATTTATGATTCTGGATTTAAAGTGCTTTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT154686 WP_001277107.1 NZ_CP051661:c2377800-2377534 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT154686 NZ_CP068216:c815563-815270 [Staphylococcus pasteuri]
GTGACAGAACAAGATCATGCACATCACTCAGAACAAATTAAAACAAATTTGAAATCGAGATTAAATAGAATTGAAGGACA
AGTTCGTGCTATCAACCGAATGATAGAAGAAGATGTTTATTGTGATGATGTTTTAACACAAATTAGAGCAACACGTTCAG
CTTTAAATAGTGTAGCTACTAAATTATTAGAACAACATATGAAGAGTTGTATAACTGAAAAAGTCAATAATGGTCATCAA
GAAGAAGCCATGGAAGAGTTACTTGTAACTTTTCAAAAATTAATGAAAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References