Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 298114..298634 Replicon chromosome
Accession NZ_CP051659
Organism Escherichia coli strain 1162C

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag HH202_RS01465 Protein ID WP_001160964.1
Coordinates 298350..298634 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HH202_RS01460 Protein ID WP_000125566.1
Coordinates 298114..298347 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH202_RS01435 (293187) 293187..293474 + 288 WP_000203744.1 ferredoxin-like protein FixX -
HH202_RS01440 (293533) 293533..294864 + 1332 WP_001183209.1 MFS transporter -
HH202_RS01445 (294972) 294972..295502 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HH202_RS01450 (295495) 295495..297357 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
HH202_RS01455 (297549) 297549..298028 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HH202_RS01460 (298114) 298114..298347 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HH202_RS01465 (298350) 298350..298634 + 285 WP_001160964.1 CcdB family protein Toxin
HH202_RS01470 (298661) 298661..299509 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HH202_RS01475 (299516) 299516..299893 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HH202_RS01480 (299896) 299896..300717 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HH202_RS01485 (300714) 300714..301703 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HH202_RS01490 (301703) 301703..302989 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T154642 WP_001160964.1 NZ_CP051659:298350-298634 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T154642 NZ_CP068193:c2821-2552 [Acinetobacter johnsonii]
ATGAACGTGTTTTGGACACCGACTGCTTTAGACGATTTAGAGCGAATTATTGATTATATTGCCGAGCGTAATTTGGTTGC
TGCAATTGAATTGCACGCTTTGATTGAGGAAAAAACTGATTTACTTTCTCACAGTAGCCTGATGGGGCGATCCGGTCTGG
TCACTGGTACGCGTGAATTGGTGGTTCATCCGCACTATGTTGTGGTGTATGACGTTAAAGATACTGTTCGTATTTTAAGA
GTTTTGCATACATCGCAGCAGTGGAAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT154642 WP_000125566.1 NZ_CP051659:298114-298347 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT154642 NZ_CP068193:c3105-2818 [Acinetobacter johnsonii]
ATGGCAGAAGCGACTTTCACATTCCGCGTAGAGGAATCCTTGAAGAAAGAATTTTCTAGTTTTGCGAAGGATATGGACCG
TAGCGGAGCGCAATTATTGCGTGACTTCATGCGTGATTTTGTGAAGCAACAGCAAGAGGCTGCATCTTATGATGCTTGGT
ATCAAAAACAGGTCCAAATTGGCTTAGATGAAGCCAATGCAGGGAATCTTATTCCACATGAGGAAGTAAAATCTCGTTTT
GAAGCAAAACGTGAAAGCCTACTGGCTAAATTGGCAGCCAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References