Detailed information of TA system TA154640

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 105754..106589
• Replicon: chromosome
• Accession: NZ_CP051659
• Organism: Escherichia coli strain 1162C

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: HH202_RS00540
• Protein ID: WP_000854759.1
• Coordinates: 106212..106589 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: HH202_RS00535
• Protein ID: WP_001295723.1
• Coordinates: 105754..106122 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH202_RS00505 (101802)	101802..102275	+	474	WP_001350782.1	antirestriction protein	-
HH202_RS00510 (102367)	102367..102792	+	426	WP_000422741.1	transposase	-
HH202_RS00515 (102789)	102789..103139	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
HH202_RS00520 (103170)	103170..104783	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
HH202_RS00525 (104831)	104831..105307	+	477	WP_001186775.1	RadC family protein	-
HH202_RS00530 (105370)	105370..105591	+	222	WP_000692345.1	DUF987 domain-containing protein	-
HH202_RS00535 (105754)	105754..106122	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HH202_RS00540 (106212)	106212..106589	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
HH202_RS00545 (106586)	106586..107074	+	489	WP_000761690.1	DUF5983 family protein	-
HH202_RS00550 (107091)	107091..107267	+	177	WP_000839286.1	DUF957 domain-containing protein	-
HH202_RS00555 (107373)	107373..107522	+	150	Protein_109	hypothetical protein	-
HH202_RS00560 (107889)	107889..108065	+	177	Protein_110	helix-turn-helix domain-containing protein	-
HH202_RS00565 (108856)	108856..110478	+	1623	WP_001295726.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	89519..119545	30026

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T154640 WP_000854759.1 NZ_CP051659:106212-106589 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T154640 NZ_CP068190:6376-6663 [Acinetobacter johnsonii]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACACGATGTCTCTTTCGAAACGGCAAG
CCTTGTTTTTGATGATCCATTAAGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACTAAGGCGGAGCGAGATAGATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT154640 WP_001295723.1 NZ_CP051659:105754-106122 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT154640 NZ_CP068190:6650-6964 [Acinetobacter johnsonii]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAGGGAACGGTTGCTGACGATCAGCTAGATCTCTCAGATATACCTGAAATTACCGACTGGAGTAATGCTGTACGCC
ACAATCAATTCTATCGTCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTGCTTGCTTGGTTCAAAGCACAG
GGAAAAGGTTATCAAACACGTATGAATGAAATCTTACGGGATGCTATGCTCAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52