Detailed information of TA system TA154637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 11820..12355
• Replicon: plasmid pSJ7-1
• Accession: NZ_CP051657
• Organism: Escherichia coli strain SJ7

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: F7D02_RS27160
• Protein ID: WP_001270421.1
• Coordinates: 12068..12355 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: F7D02_RS27155
• Protein ID: WP_001132895.1
• Coordinates: 11820..12071 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D02_RS27125	7028..7333	-	306	WP_032162675.1	hypothetical protein	-
F7D02_RS27130	7337..7754	-	418	Protein_14	DUF1281 domain-containing protein	-
F7D02_RS27135	7635..8797	+	1163	Protein_15	DUF4113 domain-containing protein	-
F7D02_RS27140	8938..9900	-	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
F7D02_RS27145	9897..11102	-	1206	WP_000817643.1	AAA family ATPase	-
F7D02_RS27150	11473..11646	+	174	WP_001435278.1	hypothetical protein	-
F7D02_RS27155	11820..12071	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
F7D02_RS27160	12068..12355	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F7D02_RS27165	12357..12762	+	406	Protein_21	DDE-type integrase/transposase/recombinase	-
F7D02_RS27170	13408..14385	+	978	WP_000361617.1	RepB family plasmid replication initiator protein	-
F7D02_RS27175	14669..15409	-	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
F7D02_RS27180	15533..15673	-	141	Protein_24	serine/threonine protein kinase	-
F7D02_RS27185	15724..15885	+	162	Protein_25	epoxyqueuosine reductase QueH	-
F7D02_RS27190	16196..16570	-	375	WP_001368887.1	hypothetical protein	-
F7D02_RS27195	16773..16952	+	180	WP_000526860.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyB / hlyD / exeG / exeE / stcE	1..74390	74390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T154637 WP_001270421.1 NZ_CP051657:12068-12355 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T154637 NZ_CP068185:47008-47403 [Acinetobacter johnsonii]
ATGATTTACCTCTTAGACACAAATATTTGTATCTATGTCATCAACAATAAGCCTCAACAAGTTTTTGAGCGCTTTAAGCA
ATATCAACTCGGCCAGCTTGCGATTTCGAGTATCACGGCATCTGAGCTGGCTTTTGGTGTAGAGAAGTCTGGATCTGAAC
GCAATAAACAGGCGTTAAACAAGTTTTTGTCACCTTTAGAGATTCTTCCTTACGATGAACAGGCGATTTGGCATTACGCC
AAATTACGCCAGAATCTTCAATCTACAGGTAAACCGATTGGCAGCTTAGATATGCTGATCGCTGCTCATGCTTTGGCATT
GGATGTTGTATTAGTTACGAACAATACAAAAGAATTTGAACGAATTGATGGTCTGAAGCTAGAGAATTGGGTCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT154637 WP_001132895.1 NZ_CP051657:11820-12071 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT154637 NZ_CP068185:46781-47011 [Acinetobacter johnsonii]
ATGGAAATTGCGAAAGTATTTCAAACTGGTCGCAGTCAGGCTGTTCGCTTGCCTAAAGCCTTTCGCTTTAATGGCACGGA
AGTGGCCATCAAAAGCTTCGGTCGTGGTGTATTACTCATGCCCATCGACAATCCTTGGGATGTCATGTTAGAAGCAATAA
ATGAGTTTGAGGTTGGATTCCAATTGGAGCGAGCTGATCAAGGCGAACAGCTCCGTGAGGAGTTCAGATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6