Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 149131..149656 Replicon plasmid pRM11911
Accession NZ_CP051656
Organism Escherichia coli strain RM11911

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0L1BNN5
Locus tag F7D04_RS26870 Protein ID WP_032285172.1
Coordinates 149131..149436 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag F7D04_RS26875 Protein ID WP_000813639.1
Coordinates 149438..149656 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D04_RS26845 145303..145719 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
F7D04_RS26850 145712..146692 - 981 WP_077166745.1 plasmid segregation protein ParM -
F7D04_RS26855 147106..147414 - 309 WP_000030199.1 hypothetical protein -
F7D04_RS26860 147501..148145 - 645 WP_001144036.1 ParA family protein -
F7D04_RS26865 148324..149130 - 807 WP_063121642.1 site-specific integrase -
F7D04_RS26870 149131..149436 - 306 WP_032285172.1 type II toxin-antitoxin system toxin CcdB Toxin
F7D04_RS26875 149438..149656 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F7D04_RS26880 150505..151743 + 1239 WP_050940296.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
F7D04_RS26885 151727..152554 + 828 WP_172636186.1 HAD-IIB family hydrolase -
F7D04_RS26890 152557..153573 + 1017 WP_032280058.1 Gfo/Idh/MocA family oxidoreductase -
F7D04_RS26895 153575..153844 + 270 WP_000379710.1 SemiSWEET transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyC / hlyA / hlyB / hlyD / cnf1 / cdtA / cdtB / cdtC / afaE-VIII / afaD-VIII / afaC-VIII / afaB-VIII / afaA-VIII / espP 1..175089 175089


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11708.49 Da        Isoelectric Point: 6.4674

>T154636 WP_032285172.1 NZ_CP051656:c149436-149131 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
SDLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T154636 NZ_CP068182:12854-13249 [Acinetobacter ursingii]
ATGATTTACCTCTTAGACACAAATATTTGTATCTATGTCATCAACAATAAGCCTCAACAAGTTTTTGAGCGCTTTAAGCA
ATATCAACTCGGCCAGCTTGCGATTTCGAGTATCACGGCATCTGAGCTGGCTTTTGGTGTAGAGAAGTCTGGATCTGAAC
GCAATAAACAGGCGTTAAACAAGTTTTTGTCACCTTTAGAGATTCTTCCTTACGATGAACAGGCGATTTGGCATTACGCC
AAATTACGCCAGAATCTTCAATCTACAGGTAAACCGATTGGCAGCTTAGATATGCTGATCGCTGCTCATGCTTTGGCATT
GGATGTTGTATTAGTTACGAACAATACAAAAGAATTTGAACGAATTGATGGTCTGAAGCTAGAGAATTGGGTCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT154636 WP_000813639.1 NZ_CP051656:c149656-149438 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT154636 NZ_CP068182:12627-12857 [Acinetobacter ursingii]
ATGGAAATTGCGAAAGTATTTCAAACTGGTCGCAGTCAGGCTGTTCGCTTGCCTAAAGCCTTTCGCTTTAATGGCACGGA
AGTGGCCATCAAAAGCTTCGGTCGTGGTGTATTACTCATGCCCATCGACAATCCTTGGGATGTCATGTTAGAAGCAATAA
ATGAGTTTGAGGTTGGATTCCAATTGGAGCGAGCTGATCAAGGCGAACAGCTCCGTGAGGAGTTCAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L1BNN5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References