Detailed information of TA system TA154635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 62070..62626
• Replicon: plasmid pRM11911
• Accession: NZ_CP051656
• Organism: Escherichia coli strain RM11911

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: F7D04_RS26470
• Protein ID: WP_050939058.1
• Coordinates: 62351..62626 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: F7D04_RS26465
• Protein ID: WP_050939060.1
• Coordinates: 62070..62351 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D04_RS26435	57794..58798	+	1005	WP_050939068.1	IS110 family transposase	-
F7D04_RS26440	59049..59714	-	666	WP_072095473.1	transposase	-
F7D04_RS26445	59749..59907	+	159	WP_077793267.1	toxin-antitoxin system protein	-
F7D04_RS26450	59904..60181	+	278	Protein_69	type II toxin-antitoxin system RelE/ParE family toxin	-
F7D04_RS26455	60227..60694	+	468	WP_050939063.1	DNA polymerase III subunit epsilon	-
F7D04_RS26460	61019..61477	+	459	WP_050939085.1	hypothetical protein	-
F7D04_RS26465	62070..62351	+	282	WP_050939060.1	ribbon-helix-helix protein, CopG family	Antitoxin
F7D04_RS26470	62351..62626	+	276	WP_050939058.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F7D04_RS26475	62732..63013	+	282	WP_050939055.1	hypothetical protein	-
F7D04_RS26480	63250..63501	-	252	WP_050939053.1	hypothetical protein	-
F7D04_RS26485	64108..65260	-	1153	Protein_76	IS3 family transposase	-
F7D04_RS26490	65319..65561	+	243	Protein_77	transposase	-
F7D04_RS26495	66826..67344	+	519	WP_050939047.1	alpha-hemolysin-activating lysine-acyltransferase HlyC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / cnf1 / cdtA / cdtB / cdtC / afaE-VIII / afaD-VIII / afaC-VIII / afaB-VIII / afaA-VIII / espP	1..175089	175089

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10753.44 Da
• Isoelectric Point: 9.9324

>T154635 WP_050939058.1 NZ_CP051656:62351-62626 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFETREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T154635 NZ_CP068180:c3216471-3216151 [Acinetobacter ursingii]
ATGTTATTTATTGAAACCAGTATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAAGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGTAAAGTACGTTGTGTTCAAGGTA
ACAAAGGGAAAAGTGGTGGCATACGGGTGATTTATTATTGGGTTACCGAGTATGATCAGATCTTTTTCTTAGTGGCTTAT
CCCAAATCAGTGAAAGATAATTTAACAGATAAGGAAACAGCCATGCTGCGTCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10335.74 Da
• Isoelectric Point: 6.4601

>AT154635 WP_050939060.1 NZ_CP051656:62070-62351 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEERNRLTLEALDDVTSGQVIDHQAVQAWA
DSLSTDNPLSVPR

Nucleotide

• Length: 282 bp

>AT154635 NZ_CP068180:c3216158-3215886 [Acinetobacter ursingii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTGAAGAAGTTAAAGCAAG
TCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTAAGCCAAAATGAATTTGCGGCAC
GTCTGCATATTAGTCCTCGTACCTTGCAAAATTGGGAACAGGGGCGACGCTATCCAACGGGGCCAGCAGTGACATTAATT
CGAATTCTAGATGCTCACCCAAGCCTTATTTGA