Detailed information of TA system TA154634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 43209..43834
• Replicon: plasmid pRM11911
• Accession: NZ_CP051656
• Organism: Escherichia coli strain RM11911

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F7D04_RS26360
• Protein ID: WP_000911315.1
• Coordinates: 43209..43607 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: F7D04_RS26365
• Protein ID: WP_000450532.1
• Coordinates: 43607..43834 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D04_RS26345	39513..40022	+	510	Protein_48	conjugal transfer entry exclusion protein TraS	-
F7D04_RS26350	40036..40767	+	732	WP_000850423.1	complement resistance protein TraT	-
F7D04_RS26355	41020..43200	+	2181	WP_069903968.1	type IV conjugative transfer system coupling protein TraD	-
F7D04_RS26360	43209..43607	-	399	WP_000911315.1	tRNA(fMet)-specific endonuclease VapC	Toxin
F7D04_RS26365	43607..43834	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / cnf1 / cdtA / cdtB / cdtC / afaE-VIII / afaD-VIII / afaC-VIII / afaB-VIII / afaA-VIII / espP	1..175089	175089

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14871.17 Da
• Isoelectric Point: 8.5264

>T154634 WP_000911315.1 NZ_CP051656:c43607-43209 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQARPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T154634 NZ_CP068179:1225-1566 [Acinetobacter ursingii]
ATGCCTCAAAATTATGTACCAGAAGAAGGCGATATTATCTGGATTAATTTTAATCCACAGAGTGGGCATGAACAGGCAGG
ACATAGACCTGCCTTAGTTTTAAGTCCTTCATCTTATAATAAAGCCACAAGCCTACTAGTTTGCTGTCCAATGACAACGC
AGATAAAAGGGTATCCTTTCGAAGTTAAAATTGTAGGAAATCCTGAAAATGTAGCACTATCTGATCAAGTAAAAAGTTTA
GATTGGGTCGCTCGATCAGCAACGAAAAAGGGACAAGCTAAATCTGAAGAACTTGAGGAAGTGCGACAAAAAATTGCAGC
CTTACTGAAAATATCAACTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT154634 WP_000450532.1 NZ_CP051656:c43834-43607 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT154634 NZ_CP068179:986-1225 [Acinetobacter ursingii]
ATGGAACTAGCTATTAAAAAGTGGGGCAATAGTGCTGCTGTCCGCCTACCATCCATTTTATTAGAAAGCCTTAATCTTAA
ATTGGATAGCCGTGTTGAGGTATTTACTCAAGATGAAGCGATCGTTATCAAGCCAATTAAATCAAAAATATCTTTAGAGC
AGCTTTTAGCAGATATAACATCTGATAATTTGCATTGTGAAGTGGATACTGGACATGCTGTAGGTAAGGAAATCTATTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5