Detailed information of TA system TA154624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 37234..37859
• Replicon: plasmid pRM13745-2
• Accession: NZ_CP051655
• Organism: Escherichia coli strain RM13745

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F7D05_RS26275
• Protein ID: WP_000911315.1
• Coordinates: 37234..37632 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: F7D05_RS26280
• Protein ID: WP_000450532.1
• Coordinates: 37632..37859 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D05_RS26260	33538..34047	+	510	Protein_37	conjugal transfer entry exclusion protein TraS	-
F7D05_RS26265	34061..34792	+	732	WP_000850423.1	complement resistance protein TraT	-
F7D05_RS26270	35045..37225	+	2181	WP_069903968.1	type IV conjugative transfer system coupling protein TraD	-
F7D05_RS26275	37234..37632	-	399	WP_000911315.1	tRNA(fMet)-specific endonuclease VapC	Toxin
F7D05_RS26280	37632..37859	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyB / hlyD / hlyD	1..70650	70650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14871.17 Da
• Isoelectric Point: 8.5264

>T154624 WP_000911315.1 NZ_CP051655:c37632-37234 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQARPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T154624 NZ_CP068165:875875-876129 [Corynebacterium aurimucosum]
ATGATTCTTTCGTGGACAGAGGAAGCGTGGGAGGACTACCTCTGGTGGCAAGCTCACAACAAGAAGATCCTCAAGAGAAT
CAATAGCCTCATTAAAGACATTGCACGAAACGGCAATGAAGGGATAGGAAAACCCGAACCACTCAAGTTCGAGTTAGCCG
GGTTCTGGTCTCGGCGAATTACGGAAGAGCACCGACTGATCTATCGCATTAAGGGCGTGACCATCGAGATTGCTGGGTGC
AGATACCACTACTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT154624 WP_000450532.1 NZ_CP051655:c37859-37632 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT154624 NZ_CP068165:875612-875869 [Corynebacterium aurimucosum]
ATGAAAACCATGTCCTACTCGGAATCGCGAGCAAGATACGCCGAGGTCCTACAGTCAGTCGTTGATGACCGTGAAGAAGT
CATCATCACTCGCAGTGGTCATGAGTCTGCAGTCATCGTTTCCTTAGATGACTACAACTCTCTTCGCGAAACCGCTTACC
TTCTGCGTTCCCCTCGCAATGCGCAGCGCCTGCTTGACTCCATCGAGAGTCTCAACACCGGGGAAGCAACGGAGCACGAA
CTCATTGAGGAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5