Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 34198..34723 Replicon plasmid pRM13745-1
Accession NZ_CP051654
Organism Escherichia coli strain RM13745

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0L1BNN5
Locus tag F7D05_RS26005 Protein ID WP_032285172.1
Coordinates 34198..34503 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag F7D05_RS26010 Protein ID WP_000813639.1
Coordinates 34505..34723 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D05_RS25980 30370..30786 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
F7D05_RS25985 30779..31759 - 981 WP_077166745.1 plasmid segregation protein ParM -
F7D05_RS25990 32173..32481 - 309 WP_000030199.1 hypothetical protein -
F7D05_RS25995 32568..33212 - 645 WP_001144036.1 ParA family protein -
F7D05_RS26000 33391..34197 - 807 WP_063121642.1 site-specific integrase -
F7D05_RS26005 34198..34503 - 306 WP_032285172.1 type II toxin-antitoxin system toxin CcdB Toxin
F7D05_RS26010 34505..34723 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F7D05_RS26015 35572..36810 + 1239 WP_050940296.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
F7D05_RS26020 36794..37621 + 828 WP_172636186.1 HAD-IIB family hydrolase -
F7D05_RS26025 37624..38635 + 1012 Protein_42 Gfo/Idh/MocA family oxidoreductase -
F7D05_RS26030 38637..38905 + 269 Protein_43 SemiSWEET transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - afaE-VIII / afaD-VIII / afaC-VIII / afaB-VIII / afaA-VIII / espP / espP 1..45063 45063


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11708.49 Da        Isoelectric Point: 6.4674

>T154623 WP_032285172.1 NZ_CP051654:c34503-34198 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
SDLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T154623 NZ_CP068165:875612-875869 [Corynebacterium aurimucosum]
ATGAAAACCATGTCCTACTCGGAATCGCGAGCAAGATACGCCGAGGTCCTACAGTCAGTCGTTGATGACCGTGAAGAAGT
CATCATCACTCGCAGTGGTCATGAGTCTGCAGTCATCGTTTCCTTAGATGACTACAACTCTCTTCGCGAAACCGCTTACC
TTCTGCGTTCCCCTCGCAATGCGCAGCGCCTGCTTGACTCCATCGAGAGTCTCAACACCGGGGAAGCAACGGAGCACGAA
CTCATTGAGGAAGACTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT154623 WP_000813639.1 NZ_CP051654:c34723-34505 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT154623 NZ_CP068165:875875-876129 [Corynebacterium aurimucosum]
ATGATTCTTTCGTGGACAGAGGAAGCGTGGGAGGACTACCTCTGGTGGCAAGCTCACAACAAGAAGATCCTCAAGAGAAT
CAATAGCCTCATTAAAGACATTGCACGAAACGGCAATGAAGGGATAGGAAAACCCGAACCACTCAAGTTCGAGTTAGCCG
GGTTCTGGTCTCGGCGAATTACGGAAGAGCACCGACTGATCTATCGCATTAAGGGCGTGACCATCGAGATTGCTGGGTGC
AGATACCACTACTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L1BNN5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References