Detailed information of TA system TA154617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 59364..59989
• Replicon: plasmid pRM13752
• Accession: NZ_CP051653
• Organism: Escherichia coli strain RM13752

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F7D06_RS26135
• Protein ID: WP_000911315.1
• Coordinates: 59364..59762 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: F7D06_RS26140
• Protein ID: WP_000450532.1
• Coordinates: 59762..59989 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D06_RS26120	55668..56177	+	510	Protein_62	conjugal transfer entry exclusion protein TraS	-
F7D06_RS26125	56191..56922	+	732	WP_000850423.1	complement resistance protein TraT	-
F7D06_RS26130	57175..59355	+	2181	WP_069903968.1	type IV conjugative transfer system coupling protein TraD	-
F7D06_RS26135	59364..59762	-	399	WP_000911315.1	tRNA(fMet)-specific endonuclease VapC	Toxin
F7D06_RS26140	59762..59989	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyC / hlyA / hlyB / hlyD / cnf1	1..99009	99009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14871.17 Da
• Isoelectric Point: 8.5264

>T154617 WP_000911315.1 NZ_CP051653:c59762-59364 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQARPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T154617 NZ_CP068159:1546046-1546327 [Corynebacterium riegelii]
ATGATCCAGTCGTTCGCCAACAAGGACACTGAGCGTTTGTGGAATCGAGAGCGTGTGCCCTCGATCGATTCCCGCATCCA
CTCAGTGGCGCTGCGTAAATTGCGTCAGCTTGGCTACGCGCAGAGCCTTCAAGAGTTGCGGATTCCGCCGGGAAACCGTC
TCGAGGCGTTGAAAGGGGATAGGCGGGGTCAGCACAGTATTCGCATCAATAGCCAGTGGCGGATCTGCTTTCGGTGGACG
GCAGCTGGTCCAGAGGAGGTCGAGATTGTTGACTATCACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT154617 WP_000450532.1 NZ_CP051653:c59989-59762 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT154617 NZ_CP068159:1546314-1546622 [Corynebacterium riegelii]
TTGACTATCACTGACAAGCTTCCACCAGTACACCCTGGTGAAATTCTAATGGAGGACTTCCTCGAGGAGATGGGGATAAC
GCAGCACAAGCTTGCTGTTTCTATCGGCGTCCCCCCTCGCCGGATCAACGAGATCGTCCACGGGAAGCGCGCTGTTACTG
CCGATACTGCACTCCGTTTAGGGAAGTATTTCGGAATGAGCCCCCAGTTCTGGCTCGGGTTACAGACCCAGTACGACTTG
GACGTGGCAGAGGATAAAATCCTTGCCGAGATTGAGCGGATTCAGCCGCTGCAGGCTGCCTCGGCATAG

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5