Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20987..21512 Replicon plasmid pRM13752
Accession NZ_CP051653
Organism Escherichia coli strain RM13752

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0L1BNN5
Locus tag F7D06_RS25900 Protein ID WP_032285172.1
Coordinates 20987..21292 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag F7D06_RS25905 Protein ID WP_000813639.1
Coordinates 21294..21512 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D06_RS25875 17159..17575 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
F7D06_RS25880 17568..18548 - 981 WP_077166745.1 plasmid segregation protein ParM -
F7D06_RS25885 18962..19270 - 309 WP_000030199.1 hypothetical protein -
F7D06_RS25890 19357..20001 - 645 WP_001144036.1 ParA family protein -
F7D06_RS25895 20180..20986 - 807 WP_063121642.1 site-specific integrase -
F7D06_RS25900 20987..21292 - 306 WP_032285172.1 type II toxin-antitoxin system toxin CcdB Toxin
F7D06_RS25905 21294..21512 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F7D06_RS25910 22361..23599 + 1239 WP_050940296.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
F7D06_RS25915 23583..24410 + 828 WP_172636186.1 HAD-IIB family hydrolase -
F7D06_RS25920 24413..25429 + 1017 WP_032280058.1 Gfo/Idh/MocA family oxidoreductase -
F7D06_RS25925 25431..25700 + 270 WP_000379710.1 SemiSWEET transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP / hlyC / hlyA / hlyB / hlyD / cnf1 1..99009 99009


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11708.49 Da        Isoelectric Point: 6.4674

>T154616 WP_032285172.1 NZ_CP051653:c21292-20987 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
SDLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T154616 NZ_CP068159:c953549-953241 [Corynebacterium riegelii]
GTGGAGATCATTTCCAGCAGCTTGTTTGACGCCTGGTTAGGTGAACTAAAAGACAAACATGCTCAGCGGCGCATTTTGTA
TGCAATAGCACGATGCGAAGCACACGGAAGAATGATTGGAGACATCAAACCCGTTGGCGAGAAGGTTAGCGAGATGAGAT
TTCATTTCGGGCCCGGCTATCGGGTGTACTACACCCGACTAGGCGCAATAACGGTTTTCCTGCTGGCCGGAGGAGATAAA
TCCAGCCAGGATAAAGATATTCGAACCGCTCAAAAGCTCGCACAACAGGTAAGGGAGGAACAGTCGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT154616 WP_000813639.1 NZ_CP051653:c21512-21294 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT154616 NZ_CP068159:c953244-952951 [Corynebacterium riegelii]
GTGAAGGAAGTAACATTTTCAGCGTTCGACGCAAGCAAATACCTCGATAGCCAAGAAGCAATGAACGATTACCTGGCTAT
CGCACTCGAAGACGGCGATACGAAAACCGTACAGGTGGTTTTGCGTGACATTGCAAGAGCACACGGCATGAGCCAGCTCG
CTAAAGAGACTCAACTCAACCGTGAATCCCTCTACAAGTCACTATCAAAAGACGGTAACCCGTCCTTTGCGGCTATCACG
AAAATCATGAAAGCCCTAGGGCTCAAAATAACTCTCGCTACACAAAATGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L1BNN5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References